Thrombin is present in the lungs of patients with primary extremity osteosarcoma and pulmonary metastases

被引:9
作者
Hernández-Rodríguez, NA
Correa, E
Sotelo, R
Gómez-Ruiz, C
Contreras-Paredes, A
Green, L
机构
[1] Inst Nacl Cancerol, Subdirecc Invest Clin, Mexico City 14000, DF, Mexico
[2] Inst Nacl Cancerol, Serv Neumol, Mexico City 14000, DF, Mexico
[3] Inst Nacl Cancerol, Dept Citopatol, Mexico City 14000, DF, Mexico
关键词
thrombin; fibrinogen; pulmonary metastasis; osteosarcoma;
D O I
10.1177/172460080201700308
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Osteosarcoma is a rare cancer, which metastasizes to the lung in up to 80% of cases. Thrombin is involved in metastasis and is present in the lungs of patients with pulmonary metastases (PM). To identify its role in PM and osteosarcoma, we measured thrombin levels in the bronchoalveolar lavage fluid (BALF) of 15 patients. BALF was collected at different stages of the disease and correlated with the diagnosis of PM. We also assessed fibrinogen overexpression in the tumors. We found that 11/15 (73%) patients with high thrombin levels in the lungs developed PM within the first 12 months from primary surgery. The median thrombin concentration in the BALF of these patients increased up to 8x10(-9) M (range, 3x10(-9)M(-)15x10(-9)M), which represents a more than 100-fold increase compared to patients without PM (p<0.0001). Eight of 15 (53%) primary and 11/15 (73%) metastatic samples showed fibrinogen overexpression. A significant difference between high thrombin levels, fibrinogen overexpression and PM was found compared to patients without PM (p=0.00073 and p=0.025). These results show that thrombin levels are increased in the lungs of patients with primary osteosarcoma and a high risk of developing PM. They suggest that thrombin may be involved in the development of PM.
引用
收藏
页码:189 / 195
页数:7
相关论文
共 29 条
  • [1] Evidence that thrombin-stimulated DNA synthesis in pulmonary arterial fibroblasts involves phosphatidylinositol 3-kinase-dependent p70 ribosomal S6 kinase activation
    Belham, CM
    Scott, PH
    Twomey, DP
    Gould, GW
    Wadsworth, RM
    Plevin, R
    [J]. CELLULAR SIGNALLING, 1997, 9 (01) : 109 - 116
  • [2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [3] Buckley JD, 1998, CANCER, V83, P1440, DOI 10.1002/(SICI)1097-0142(19981001)83:7<1440::AID-CNCR23>3.0.CO
  • [4] 2-3
  • [5] Differential expression of protease-activated receptors-1 and-2 in stromal fibroblasts of normal, benign, and malignant human tissues
    D'Andrea, MR
    Derian, CK
    Santulli, RJ
    Andrade-Gordon, P
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 2001, 158 (06) : 2031 - 2041
  • [6] Tumor cell invasion is promoted by activation of protease activated receptor-1 in cooperation with the αvβ5 integrin
    Even-Ram, SC
    Maoz, M
    Pokroy, E
    Reich, R
    Katz, BZ
    Gutwein, P
    Altevogt, P
    Bar-Shavit, R
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (14) : 10952 - 10962
  • [7] Falanga A, 1998, THROMB HAEMOSTASIS, V79, P23
  • [8] UNDERSTANDING THROMBIN AND HEMOSTASIS
    FENTON, JW
    OFOSU, FA
    BREZNIAK, DV
    HASSOUNA, HI
    [J]. HEMATOLOGY-ONCOLOGY CLINICS OF NORTH AMERICA, 1993, 7 (06) : 1107 - 1119
  • [9] NEW ASPECTS OF CELL BIOLOGY IN OSTEOSARCOMA
    GRUNDMANN, E
    UEDA, Y
    SCHNEIDERSTOCK, R
    ROESSNER, A
    [J]. PATHOLOGY RESEARCH AND PRACTICE, 1995, 191 (06) : 563 - 570
  • [10] BRONCHOALVEOLAR LAVAGE FLUID CELL COUNTS IN CRYPTOGENIC FIBROSING ALVEOLITIS AND THEIR RELATION TO THERAPY
    HASLAM, PL
    TURTON, CWG
    LUKOSZEK, A
    SALSBURY, AJ
    DEWAR, A
    COLLINS, JV
    TURNERWARWICK, M
    [J]. THORAX, 1980, 35 (05) : 328 - 339