Measuring cholesterol in macrophages: Comparison of high-performance liquid chromatography and gas-liquid chromatography with enzymatic fluorometry

被引：10

作者：

Cullen, P ^{[1
]}

Tegelkamp, K ^{[1
]}

Fobker, M ^{[1
]}

Kannenberg, F ^{[1
]}

Assmann, G ^{[1
]}

机构：

[1] INST KLIN CHEM & LAB MED, ZENT LAB, D-48149 MUNSTER, GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 251卷 / 01期

关键词：

D O I：

10.1006/abio.1997.2227

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol and cholesteryl esters in human macrophages were analyzed by three different methods. Values obtained by high-performance liquid chromatography and by gas-liquid chromatography were compared with those obtained using enzymatic fluorometry. We also assessed fractional lipid recovery from these cells using radiolabeled cholesterol and cholesteryl ester. Enzymatic fluorometry substantially underestimated cellular cholesterol content. Two reasons for this were found. First, recovery into a variety of solvents was incomplete, particularly when extracted lipids were dried and redissolved in a second solvent. Second, the cells appeared to contain an intrinsic inhibitor of the enzymatic fluorometric method. (C) 1997 Academic Press.

引用

页码：39 / 44

页数：6

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