Different expression of adenylyl cyclase isoforms after retinoic acid induction of P19 teratocarcinoma cells

被引：16

作者：

Lipskaia, L ^{[1
]}

Djiane, A ^{[1
]}

Defer, N ^{[1
]}

Hanoune, J ^{[1
]}

机构：

[1] HOP HENRI MONDOR,INSERM,U99,F-94010 CRETEIL,FRANCE

来源：

FEBS LETTERS | 1997年 / 415卷 / 03期

关键词：

adenylyl cyclase isoform; cell differentiation; retinoic acid;

D O I：

10.1016/S0014-5793(97)01001-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the adenylyl cyclase (AC) activity and gene expression in retinoic acid (RA)-primed murine P19 teratocarcinoma cells, which recapitulate in vitro the first stages of neuroectodermal formation. Here we show that the P19 stem cells possess a basal Ca2+/CaM-stimulated AC activity, which increases about 10-fold after RA induction. The rise of AC activity is associated with a stage-specific up-regulation of AC2, AC5 and AC8 mRNAs and a down-regulation of AC3 mRNA. P19 cells provide a powerful model to investigate the role and specific regulation of AC isoforms during neuronal differentiation. (C) 1997 Federation of European Biochemical Societies.

引用

页码：275 / 280

页数：6

共 40 条

[1] COMPARISON OF 3 ACTIN-CODING SEQUENCES IN THE MOUSE - EVOLUTIONARY RELATIONSHIPS BETWEEN THE ACTIN GENES OF WARM-BLOODED VERTEBRATES [J].