Virus-induced silencing of FtsH gene in Nicotiana benthmiana causes a striking bleached leaf phenotype

被引：18

作者：

Saitoh, H

Terauchi, R

机构：

[1] Iwate Biotechnol Res Ctr, Kitakami, Iwate 0240003, Japan

[2] Japan Soc Promot Sci, Chiyoda Ku, Tokyo 1028471, Japan

来源：

GENES & GENETIC SYSTEMS | 2002年 / 77卷 / 05期

关键词：

D O I：

10.1266/ggs.77.335

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant Potato virus X (PVX) vector, pTXS.FtsH, harboring partial sequence of FtsH gene of Nicotiana benthamiana was constructed to silence the expression of endogenous FtsH homologous gene in N. benthamiana. Inoculation with in vitro runoff transcript of pTXS.FtsH to N. benthamiana plants allowed silencing of FtsH, causing striking bleaching of upper leaves reminiscent of var2 mutant phenotype of Arabidopsis thaliana. FtsH-silenced plants exhibited no resistance against Tobacco mosaic virus (TAW) and a phytopathogenic fungus Botrytis cinerea. Virus-induced gene silencing (VIGS) of N. benthamiana with PVX as demonstrated here would be an efficient and rapid method to study the function of other elements of photosystem II (PSII) in planta.

引用

收藏

页码：335 / 340

页数：6

相关论文

共 17 条

[1] A critical role for the Var2 FtsH homologue of Arabidopsis thaliana in the photosystem II repair cycle in vivo [J].

Bailey, S ;

Thompson, E ;

Nixon, PJ ;

Horton, P ;

Mullineaux, CW ;

Robinson, C ;

Mann, NH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (03) :2006-2011

[2] Fast forward genetics based on virus-induced gene silencing [J].

Baulcombe, DC .

CURRENT OPINION IN PLANT BIOLOGY, 1999, 2 (02) :109-113

[3] JELLYFISH GREEN FLUORESCENT PROTEIN AS A REPORTER FOR VIRUS-INFECTIONS [J].

BAULCOMBE, DC ;

CHAPMAN, S ;

CRUZ, SS .

PLANT JOURNAL, 1995, 7 (06) :1045-1053

[4] Virus-induced silencing of a plant cellulose synthase gene [J].

Burton, RA ;

Gibeaut, DM ;

Bacic, A ;

Findlay, K ;

Roberts, K ;

Hamilton, A ;

Baulcombe, DC ;

Fincher, GB .

PLANT CELL, 2000, 12 (05) :691-705

[5] Mapping of host range restriction of the rakkyo strain of tobacco mosaic virus in Nicotiana tabacum cv bright yellow [J].

Chen, JB ;

Watanabe, Y ;

Sako, N ;

Ohshima, K ;

Okada, Y .

VIROLOGY, 1996, 226 (02) :198-204

[6] Mutations in the Arabidopsis VAR2 locus cause leaf variegation due to the loss of a chloroplast FtsH protease [J].

Chen, M ;

Choi, YD ;

Voytas, DF ;

Rodermel, S .

PLANT JOURNAL, 2000, 22 (04) :303-313

[7] A 200-AMINO ACID ATPASE MODULE IN SEARCH OF A BASIC FUNCTION [J].

CONFALONIERI, F ;

DUGUET, M .

BIOESSAYS, 1995, 17 (07) :639-650

[8] Identification, characterization, and molecular cloning of a homologue of the bacterial FtsH protease in chloroplasts of higher plants [J].

Lindahl, M ;

Tabak, S ;

Cseke, L ;

Pichersky, E ;

Andersson, B ;

Adam, Z .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (46) :29329-29334

[9] The thylakoid FtsH protease plays a role in the light-induced turnover of the photosystem II D1 protein [J].

Lindahl, M ;

Spetea, C ;

Hundal, T ;

Oppenheim, AB ;

Adam, Z ;

Andersson, B .

PLANT CELL, 2000, 12 (03) :419-431

[10] Photosystem-II damage and repair cycle in chloroplasts:: what modulates the rate of photodamage in vivo? [J].

Melis, A .

TRENDS IN PLANT SCIENCE, 1999, 4 (04) :130-135