Detection of minor drug-resistant populations by parallel allele-specific sequencing

被引：58

作者：

Cai, Fangping

Chen, Haifeng

Hicks, Charles B.

Bartlett, John A.

Zhu, Jun

Gao, Feng ^{[1
]}

机构：

[1] Duke Univ, Med Ctr, Duke Human Vaccine Inst, Durham, NC 27710 USA

[2] Duke Univ, Med Ctr, Dept Med, Div Infect Dis, Durham, NC 27710 USA

[3] Duke Univ, Med Ctr, Inst Genome Sci & Policy, Durham, NC 27710 USA

[4] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 02期

关键词：

D O I：

10.1038/nmeth995

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at similar to 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.

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页码：123 / 126

页数：4

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