Inhibition of a photosystem II electron transfer reaction by the natural product sorgoleone

被引:101
作者
Gonzalez, VM
Kazimir, J
Nimbal, C
Weston, LA
Cheniae, GM
机构
[1] UNIV KENTUCKY,DEPT HORT & LANDSCAPE ARCHITECTURE,LEXINGTON,KY 40546
[2] UNIV KENTUCKY,DEPT AGRON,LEXINGTON,KY 40546
关键词
allelopathy; sorgoleone; photosystem II; electron transfer; herbicide; SORGHUM SORGHUM-BICOLOR; WATER-OXIDIZING ENZYME; STRIGA-ASIATICA; ROOT EXUDATE; O-2; EVOLUTION; SYSTEM-II; CHLOROPLASTS; GERMINATION; BINDING; TRYPSIN;
D O I
10.1021/jf960733w
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Effects of the alleochemical sorgoleone on photosynthetic electron transport by oxygen-evolving chloroplast thylakoids and Triton X-100-prepared Photosystem II (PSII) membranes were analyzed. The Hill activity of the thylakoids proved to be at least as sensitive to inhibition by sorgoleone as it was to DCMU [3-(3,4-dichlorophenyl)-1,1-dimethylurea], a potent herbicidal inhibitor of PSII. However, a Photosystem I (PSI) partial reaction was not affected by a 10-fold greater concentration of sorgoleone than is required for complete inhibition of Hill activity. Measurements of flash-induced chlorophyll a variable fluorescence showed that sorgoleone neither dissipated excitation energy nor diminished the amplitude of chlorophyll a variable fluorescence. However, it inhibited the decay of variable fluorescence as effectively as DCMU, which blocks the oxidation of the PSII-reduced primary electron acceptor, Q(A)(-), by the PSII secondary electron acceptor, Q(B), by displacing Q(B) from the D-1 protein. Additionally, sorgoleone competitively inhibited the binding of [C-14]atrazine to the Q(B) locus. Increasing durations of trypsin proteolysis of the PSII membranes or thylakoids and of the Q(B)-binding niche itself caused parallel losses of inhibition of O-2 evolution from sorgoleone and DCMU, as well as from bromoxynil, a phenol-type herbicide also binding to the Q(B) locus.
引用
收藏
页码:1415 / 1421
页数:7
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