Raman signature from brain hippocampus could aid Alzheimer's disease diagnosis

被引：54

作者：

Chen, Pu ^{[1
]}

Shen, Aiguo ^{[1
]}

Zhao, Wei ^{[2
]}

Baek, Seong-Joon ^{[3
]}

Yuan, Hua ^{[4
]}

Hu, Jiming ^{[1
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Coll Elect Informat, Wuhan 430072, Peoples R China

[3] Chonnam Natl Univ, Dept Elect & Comp Engn, Kwangju 500757, South Korea

[4] Wuhan Univ, Coll Basic Med, Wuhan 430071, Peoples R China

来源：

APPLIED OPTICS | 2009年 / 48卷 / 24期

基金：

中国国家自然科学基金;

关键词：

SECONDARY STRUCTURE; AMYLOID-BETA; SPECTROSCOPY; TISSUE; CHOLESTEROL; PROTEINS;

D O I：

10.1364/AO.48.004743

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Micro-Raman spectroscopy (MRS) is used for the first time to our knowledge to investigate brain hippocampus tissue from Alzheimer's disease (AD) infected rats. In situ Raman analysis of tissue sections provides distinct spectra useful for distinguishing AD from normal state. The biochemical changes of brain hippocampus tissue including the deposit of beta-amyloid (A beta) protein, the increase of cholesterol, and hyperphosphorylated tau are observed through MRS when AD occurs. A more convincing multi-Raman criterion based on single Raman peaks, and further in combination with statistical analysis of the entire Raman spectrum, is found capable of classifying brain hippocampus tissues with different pathological features. This study demonstrates the brain hippocampus is an important candidate for considering the early pathological state of AD, and Raman signatures from the brain hippocampus could aid AD diagnosis. In addition, Raman results undoubtedly confirm simultaneous changes of cholesterol and A beta in the progression of AD. (C) 2009 Optical Society of America

引用

页码：4743 / 4748

页数：6

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