Direct-view microscopy: Experimental investigation of the dependence of the optical sectioning characteristics on pinhole-array configuration

被引：23

作者：

Fewer, DT

Hewlett, SJ

McCabe, EM

Hegarty, J

机构：

[1] Department of Physics, Trinity College, University of Dublin

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1997年 / 187卷

关键词：

confocal microscopy; direct-view microscopy; experimental axial response; optical sectioning strength; light budget;

D O I：

10.1046/j.1365-2818.1997.2080772.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We present a comparison between theoretical and experimental results for the axial response to a plane mirror specimen of the direct-view microscope employing multiple-pinhole arrays. The effects of pinhole size, pinhole spacing and array geometry are investigated in detail with a view to (i) achieving good optical sectioning characteristics and (ii) maximizing the amount of light available for imaging. The implications of our results for practical systems as regards pinhole-array design and fabrication are also discussed.

引用

页码：54 / 61

页数：8

共 13 条

[1]

Abramowitz M., 1965, Handbook of Mathematical Functions, Dover Books on Mathematics

[2]

Boyde A., 1985, P ROY MICROSC SOC, V20, P131

[3] DEPTH RESPONSE OF CONFOCAL OPTICAL MICROSCOPES [J].