Wound repair by bone marrow stromal cells through growth factor production

被引:116
作者
Liu, Yongbo
Dulchavsky, Deborah S.
Gao, Xiaohua
Kwon, David
Chopp, Michael
Dulchavsky, Scott
Gautam, Subliash C.
机构
[1] Henry Ford Hlth Syst, Dept Surg, Detroit, MI USA
[2] Henry Ford Hlth Syst, Dept Neurol, Detroit, MI USA
关键词
wound healing; growth factors; TGF-beta; EGF; VEGF; bone marrow stromal cells;
D O I
10.1016/j.jss.2006.07.037
中图分类号
R61 [外科手术学];
学科分类号
摘要
We have previously shown that treatment with bone marrow stromal cells (BMSCs) augments the healing of fascial wounds in the rat. However, the biochemical mechanism by which BMSCs improve wound healing was not investigated. Growth factors have been shown to play a key role in repairing damaged tissue. In this study, we investigated whether BMSCs are capable of producing growth factors that play a critical role in healing of the damaged tissue. Growth factor expression in BMSCs stimulated with pro-inflammatory cytokines or wound superfusate was measured by RT-PCR and growth factor-specific quantitative sandwich enzyme-linked immunosorbent assay (ELISA). RT-PCR analysis demonstrated that BMSCs are capable of expressing transforming growth factor beta-1 (TGF-beta 1), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF) platelet-derived growth factor (PDGF), keratinocyte growth factor (KGF), fibroblast growth factor (FGF), and hepatocyte growth factor (HGF) constitutively or upon stimulation with LPS, IL-1 alpha, or TNF-alpha. Quantitative analysis of growth factor production by ELISA showed that BMSCs do not secrete TGF-beta 1, EGF or VEGF in response to uninjured fascia tissue superfusate; however, production of these growth factors is significantly increased when cells were stimulated with wound tissue superfusate. The ability of wound to stimulate growth factor production in BMSCs could be detected as early as day1 and lasted through day 7 after wounding. Thus, growth factor production by BMSCs in response to wound microenvionment suggests that BMSCs might augment wound healing through the responsive secretion of growth factors that enhance angiogenesis and promote wound repair. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:336 / 341
页数:6
相关论文
共 34 条
[1]  
Anstead G M, 1998, Adv Wound Care, V11, P277
[2]   Participation of bone marrow derived cells in cutaneous wound healing [J].
Badiavas, EV ;
Abedi, M ;
Butmarc, J ;
Falanga, V ;
Quesenberry, P .
JOURNAL OF CELLULAR PHYSIOLOGY, 2003, 196 (02) :245-250
[3]   Treatment of chronic wounds with bone marrow-derived cells [J].
Badiavas, EV ;
Falanga, V .
ARCHIVES OF DERMATOLOGY, 2003, 139 (04) :510-516
[4]   Growth factors in the treatment of diabetic foot ulcers [J].
Bennett, SP ;
Griffiths, GD ;
Schor, AM ;
Leese, GP ;
Schor, SL .
BRITISH JOURNAL OF SURGERY, 2003, 90 (02) :133-146
[5]   Human bone marrow stromal cell cultures conditioned by traumatic brain tissue extracts: Growth factor production [J].
Chen, XG ;
Katakowski, M ;
Li, Y ;
Lu, DY ;
Wang, L ;
Zhang, LJ ;
Chen, JL ;
Xu, YX ;
Gautam, S ;
Mahmood, A ;
Chopp, M .
JOURNAL OF NEUROSCIENCE RESEARCH, 2002, 69 (05) :687-691
[6]   Treatment of neural injury with marrow stromal cells [J].
Chopp, M ;
Li, Y .
LANCET NEUROLOGY, 2002, 1 (02) :92-100
[7]  
FERGUSON MK, 1982, SURG GYNECOL OBSTET, V154, P421
[8]   Muscle regeneration by bone marrow derived myogenic progenitors [J].
Ferrari, G ;
Cusella-De Angelis, G ;
Coletta, M ;
Paolucci, E ;
Stornaiuolo, A ;
Cossu, G ;
Mavilio, F .
SCIENCE, 1998, 279 (5356) :1528-1530
[9]  
Gomez N J, 1997, Adv Ren Replace Ther, V4, P390
[10]  
HASKAM DJ, 2002, SURG INFECT LARCH S1, V3, pS23