Exploring cancer metabolism using stable isotope-resolved metabolomics (SIRM)

被引：81

作者：

Bruntz, Ronald C. ^{[1
,2
]}

Lane, Andrew N. ^{[1
,2
]}

Higashi, Richard M. ^{[1
,2
]}

Fan, Teresa W. -M. ^{[1
,2
]}

机构：

[1] Univ Kentucky, Ctr Environm & Syst Biochem, Markey Canc Ctr, Lexington, KY 40506 USA

[2] Univ Kentucky, Dept Toxicol & Canc Biol, Lexington, KY 40506 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2017年 / 292卷 / 28期

基金：

美国国家卫生研究院;

关键词：

cancer; isotopic tracer; mass spectrometry (MS); metabolism; metabolomics; nuclear magnetic resonance (NMR); CELL LUNG-CANCER; PENTOSE-PHOSPHATE PATHWAY; FATTY-ACID SYNTHESIS; GLUTAMINE-METABOLISM; NEOADJUVANT CHEMOTHERAPY; PYRUVATE CARBOXYLATION; HUMAN GLIOBLASTOMA; PANCREATIC TUMORS; DEFICIENT CELLS; GLUCOSE;

D O I：

10.1074/jbc.R117.776054

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Metabolic reprogramming is a hallmark of cancer. The changes in metabolism are adaptive to permit proliferation, survival, and eventually metastasis in a harsh environment. Stable isotope-resolved metabolomics (SIRM) is an approach that uses advanced approaches of NMR and mass spectrometry to analyze the fate of individual atoms from stable isotope-enriched precursors to products to deduce metabolic pathways and networks. The approach can be applied to a wide range of biological systems, including human subjects. This review focuses on the applications of SIRM to cancer metabolism and its use in understanding drug actions.

引用

页码：11601 / 11609

页数：9

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