Determination of Δ9-THC in whole blood using gas chromatography-mass spectrometry

被引：32

作者：

Chu, MHC

Drummer, OH

机构：

[1] Victorian Inst Forens Med, Southbank, Vic 3006, Australia

[2] Monash Univ, Dept Forens Med, Southbank, Vic 3006, Australia

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 2002年 / 26卷 / 08期

关键词：

D O I：

10.1093/jat/26.8.575

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and reliable liquid-liquid extraction method for the determination of Δ9-tetrahydrocannabinol (THC) in whole blood utilizing gas chromatography-mass spectrometry in electron impact mode is described. The substance is derivatized with pentafluoropropionic anhydride in pentafluropropanol. The limit of detection is 0.5 ng/mL for a 1-mL specimen, with recovery greater than 70%. The intra-assay coefficient of variation (CV) is 3.1% to 5.2%, and the interassay CV is 6.4% to 9.5%, calculated at THC concentrations of 1,5, and 25 ng/mL. The accuracy is between 95 and 97%. The optimization of extraction and derivatization conditions is detailed.

引用

收藏

页码：575 / 581

页数：7

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