Functional genomics of cactus host shifts in Drosophila mojavensis

被引:82
作者
Matzkin, Luciano M. [1 ]
Watts, Thomas D. [1 ]
Bitler, Benjamin G. [1 ]
Machado, Carlos A. [1 ]
Markow, Therese A. [1 ]
机构
[1] Univ Arizona, Dept Ecol & Evolutionary Biol, Tucson, AZ 85721 USA
关键词
detoxification; Drosophila mojavensis; ecological genomics; Functional genomics; host shifts; microarray;
D O I
10.1111/j.1365-294X.2006.03102.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Understanding the genetic basis of adaptation to novel environments remains one of the major challenges confronting evolutionary biologists. While newly developed genomic approaches hold considerable promise for addressing this overall question, the relevant tools have not often been available in the most ecologically interesting organisms. Our study organism, Drosophila mojavensis, is a cactophilic Sonoran Desert endemic utilizing four different cactus hosts across its geographical range. Its well-known ecology makes it an attractive system in which to study the evolution of gene expression during adaptation. As a cactophile, D. mojavensis oviposits in the necrotic tissues of cacti, therefore exposing larvae and even adults to the varied and toxic compounds of rotting cacti. We have developed a cDNA microarray of D. mojavensis to examine gene expression associated with cactus host use. Using a population from the Baja California population we examined gene expression differences of third instar larvae when reared in two chemically distinct cactus hosts, agria (Stenocereus gummosus, native host) vs. organpipe (Stenocereus thurberi, alternative host). We have observed differential gene expression associated with cactus host use in genes involved in metabolism and detoxification.
引用
收藏
页码:4635 / 4643
页数:9
相关论文
共 67 条
[1]  
[Anonymous], GENETIC ANAL COMPLEX
[2]  
ATKINS WM, 1993, J BIOL CHEM, V268, P19188
[3]  
ATKINSON PW, 1988, GENETICS, V120, P713
[4]  
BATTERHAM P, 1984, EVOLUTION, V38, P644, DOI 10.1111/j.1558-5646.1984.tb00331.x
[5]   Differences in the effect of ethanol on fertility and viability components among laboratory strains of Drosophila melanogaster [J].
Bokor, K ;
Pecsenye, K .
HEREDITAS, 2000, 132 (03) :215-227
[6]   Comparative influence of Odh and Adh loci on alcohol tolerance in Drosophila melanogaster [J].
Bokor, K ;
Pecsenye, K .
GENETICS SELECTION EVOLUTION, 1998, 30 (05) :503-516
[7]   A comparison of normalization methods for high density oligonucleotide array data based on variance and bias [J].
Bolstad, BM ;
Irizarry, RA ;
Åstrand, M ;
Speed, TP .
BIOINFORMATICS, 2003, 19 (02) :185-193
[8]  
Bravo-Hollis H., 1978, Las Cactaceas de Mexico, VVolume 1
[9]   HOST-PLANT SHIFTS AND ADULT SURVIVAL IN THE CACTUS BREEDING DROSOPHILA-MOJAVENSIS [J].
BRAZNER, J ;
ABERDEEN, V ;
STARMER, WT .
ECOLOGICAL ENTOMOLOGY, 1984, 9 (04) :375-381
[10]   Genetic dissection of transcriptional regulation in budding yeast [J].
Brem, RB ;
Yvert, G ;
Clinton, R ;
Kruglyak, L .
SCIENCE, 2002, 296 (5568) :752-755