Resistance to both cyst and root-knot nematodes conferred by transgenic Arabidopsis expressing a modified plant cystatin

被引:135
作者
Urwin, PE [1 ]
Lilley, CJ [1 ]
McPherson, MJ [1 ]
Atkinson, HJ [1 ]
机构
[1] UNIV LEEDS, CTR PLANT BIOCHEM & BIOTECHNOL, LEEDS LS2 9JT, W YORKSHIRE, ENGLAND
关键词
D O I
10.1046/j.1365-313X.1997.12020455.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant nematodes are major pests of agriculture. Transgenic plant technology has been developed based on the use of proteinase inhibitors as nematode anti-feedants. The approach offers prospects for novel plant resistance and reduced use of environmentally damaging nematicides. A modified rice cystatin, Oc-I Delta D86, expressed as a transgene in Arabidopsis thaliana, has a profound effect on the size and fecundity of females for both Heterodera schachtii (beet-cyst nematode) and Meloidogyne incognita (root-knot nematode). No females of either species achieved the minimum size they require for egg production. Ingestion of Oc-I Delta D86 from the plant was correlated with loss of cysteine proteinase activity in the intestine thereby suppressing normal growth, as required of an effective antifeedant plant defence.
引用
收藏
页码:455 / 461
页数:7
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