Pooling cervical swabs and testing by ligase chain reaction are accurate and cost-saving strategies for diagnosis of Chlamydia trachomatis

被引:23
作者
Kapala, J
Copes, D
Sproston, A
Patel, J
Jang, D
Petrich, A
Mahony, J
Biers, K
Chernesky, M
机构
[1] Gamma Dynacare Med Labs, Brampton, ON L6T 5M3, Canada
[2] Choice Hlth Clin, Toronto, ON, Canada
[3] McMaster Univ, St Josephs Hosp, Reg Virol & Chlamydiol Lab, Hamilton, ON, Canada
关键词
D O I
10.1128/JCM.38.7.2480-2483.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Specimen pooling to achieve efficiency when testing urine specimens for Chlamydia trachomatis nucleic acids has been suggested, We pooled endocervical swabs from 1,288 women and also tested individual swabs by ligase chain reaction (LCR), Out of 53 positive specimens, pools of 4 or 8 specimens missed two positives, providing 96.2% accuracy compared to individual test results. Dilution and positive-control spiking experiments showed that negative specimens with inhibitors of LCR in the pool reduced the signal. Conversely, two extra positives, detected only through pooling, were negative by individual testing but became positive after storage, suggesting that fresh positive specimens with labile inhibitors may be positive in a pool because of dilution of inhibitors, For this population of women with a 4% prevalence of C. trachomatis infection, substantial savings in cost of reagents (55 to 63%) and technologist time (50 to 63%) made pooling strategies a desirable alternative to individual testing.
引用
收藏
页码:2480 / 2483
页数:4
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