Primed in situ (PRINS) labelling with Alu and satellite primers for rapid characterization of human chromosomes in hybrid cell lines

被引：20

作者：

Coullin, P

Andreo, B

Charlieu, JP

Candelier, JJ

Pellestor, F

机构：

[1] CNRS,UPR 9008,F-34033 MONTPELLIER,FRANCE

[2] INSERM,U178,F-94807 VILLEJUIF,FRANCE

来源：

CHROMOSOME RESEARCH | 1997年 / 5卷 / 05期

关键词：

human chromosomes; hybrid cell lines; primed in situ labelling method; specific labelling;

D O I：

10.1023/B:CHRO.0000038761.15856.4d

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primed in situ (PRINS) labelling method was developed as an alternative to classical cytogenetics and fluorescence in situ hybridization (FISH) for the characterization of interspecific somatic hybrids. Full karyotypes were performed by PRINS using Aluspecific primers to generate the painting of all human material associated with R-like banding. The representativity of individual human chromosomes was established using primers specific for discriminent alpha-satellite DNA sequences providing specific signals on the centromeres of the targeted chromosomes and corresponding spots in interphase nuclei. Using this methodology, a somatic hybrid clone was shown to be monochromosomal for the der(ll) from a t(11;22) patient.

引用

收藏

页码：307 / 312

页数：6

相关论文

共 22 条

[1] DEVELOPMENT OF HUMAN CHROMOSOME-SPECIFIC PCR PRIMERS FOR CHARACTERIZATION OF SOMATIC-CELL HYBRIDS [J].

ABBOTT, C ;

POVEY, S .

GENOMICS, 1991, 9 (01) :73-77

[2] INSITU HYBRIDIZATION BANDING OF HUMAN-CHROMOSOMES WITH ALU-PCR PRODUCTS - A SIMULTANEOUS KARYOTYPE FOR GENE-MAPPING STUDIES [J].

BALDINI, A ;

WARD, DC .

GENOMICS, 1991, 9 (04) :770-774

[3] A SURVEY OF THE GENOMIC DISTRIBUTION OF ALPHA-SATELLITE DNA ON ALL THE HUMAN-CHROMOSOMES, AND DERIVATION OF A NEW CONSENSUS SEQUENCE [J].

CHOO, KH ;

VISSEL, B ;

NAGY, A ;

EARLE, E ;

KALITSIS, P .

NUCLEIC ACIDS RESEARCH, 1991, 19 (06) :1179-1182

[4] GENERATION OF NOVEL SEQUENCE TAGGED SITES (STSS) FROM DISCRETE CHROMOSOMAL REGIONS USING ALU-PCR [J].

COLE, CG ;

GOODFELLOW, PN ;

BOBROW, M ;

BENTLEY, DR .

GENOMICS, 1991, 10 (03) :816-826

[5] ASSIGNMENT OF 112 MICROSATELLITE MARKERS TO 23 CHROMOSOME-11 SUBREGIONS DELINEATED BY SOMATIC HYBRIDS - COMPARISON WITH THE GENETIC-MAP [J].

COUILLIN, P ;

LEGUERN, E ;

VIGNAL, A ;

FIZAMES, C ;

RAVISE, N ;

DELPORTES, D ;

REGUIGNE, I ;

ROSIER, MF ;

JUNIEN, C ;

VANHEYNINGEN, V ;

WEISSENBACH, J .

GENOMICS, 1994, 21 (02) :379-387

[6]

COUILLIN P, 1990, ANN GENET-PARIS, V33, P196

[7] MAPPING OF HUMAN-CHROMOSOME 22 WITH A PANEL OF SOMATIC-CELL HYBRIDS [J].

DELATTRE, O ;

AZAMBUJA, CJ ;

AURIAS, A ;

ZUCMAN, J ;

PETER, M ;

ZHANG, F ;

HORSCAYLA, MC ;

ROULEAU, G ;

THOMAS, G .

GENOMICS, 1991, 9 (04) :721-727

[8] INSITU HYBRIDIZATION OF PCR AMPLIFIED INTER-ALU SEQUENCES FROM A HYBRID CELL-LINE [J].

DESMAZE, C ;

ZUCMAN, J ;

DELATTRE, O ;

THOMAS, G ;

AURIAS, A .

HUMAN GENETICS, 1992, 88 (05) :541-544

[9] IRRADIATION HYBRIDS FOR HUMAN CHROMOSOME-11 - CHARACTERIZATION AND USE FOR GENERATING REGION-SPECIFIC MARKERS IN 11Q14-Q23 [J].

GILLETT, GT ;

MCCONVILLE, CM ;

BYRD, PJ ;

STANKOVIC, T ;

TAYLOR, AM ;

HUNT, DM ;

WEST, LF ;

FOX, MF ;

POVEY, S ;

BENHAM, FJ .

GENOMICS, 1993, 15 (02) :332-341

[10] RAPID CHROMOSOME IDENTIFICATION BY OLIGONUCLEOTIDE-PRIMED IN-SITU DNA-SYNTHESIS (PRINS) [J].

GOSDEN, J ;

LAWSON, D .

HUMAN MOLECULAR GENETICS, 1994, 3 (06) :931-936