Testing for genetically modified organisms (GMOs): Past, present and future perspectives

被引:111
作者
Holst-Jensen, Arne [1 ]
机构
[1] Natl Vet Inst, Dept Feed & Food Safety, N-0106 Oslo, Norway
关键词
Transgenic crops; Detection; Identification; Quantification; Transformation; Quality assurance; High throughput; Validation; Unapproved GMO; Intragenics; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; EVENT-SPECIFIC-DETECTION; CAULIFLOWER MOSAIC-VIRUS; DNA FRAGMENT RATIOS; QUANTITATIVE PCR; MODIFIED MAIZE; MULTIPLEX-PCR; TRANSGENIC MAIZE; DETECTION SYSTEM;
D O I
10.1016/j.biotechadv.2009.05.025
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This paper presents an overview of GMO testing methodologies and how these have evolved and may evolve in the next decade. Challenges and limitations for the application of the test methods as well as to the interpretation of results produced with the methods are highlighted and discussed. bearing in mind the various interests and competences of the involved stakeholders To better understand the suitability and limitations of detection methodologies the evolution of transformation processes for creation of GMOs is briefly reviewed. (C) 2009 Elsevier Inc. All rights reserved
引用
收藏
页码:1071 / 1082
页数:12
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