Genetic variation of Chinese PRRSV strains based on ORF5 sequence

被引：48

作者：

Chen, Jian

Liu, Tao

Zhu, Cheng-Gang

Jin, Yong-Feng

Zhang, Yao-Zhou ^{[1
]}

机构：

[1] Zhejiang Sci Tech Univ, Inst Biochem, Hangzhou 310018, Peoples R China

[2] Zhejiang Univ, Coll Life Sci, Hangzhou 310029, Peoples R China

来源：

BIOCHEMICAL GENETICS | 2006年 / 44卷 / 9-10期

基金：

浙江省自然科学基金;

关键词：

PRRSV; ORF5; RT-PCR; phylogenetic analysis; genetic variation;

D O I：

10.1007/s10528-006-9039-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thirteen isolates of porcine reproductive and respiratory syndrome virus (PRRSV) from different provinces of China were studied and compared with several PRRSV isolates from other countries. Phylogenetic analysis shows that all Chinese isolates of PRRSV in this study belong to the American genotype, except for one strain, B13, which clustered as a European genotype. Sequence analysis revealed that PRRSV Chinese isolates of the American genotype were highly similar in the ORF5 sequence and could be classified into two subclades. One contains PRRSV isolates that are more closely related to the American vaccine strain MLV Resp and its parent strain VR-2332, and the other contains ones only distantly related to them. Within the Chinese isolates slight genetic variation occurred, and some strains may originate directly from the vaccine virus.

引用

页码：425 / 435

页数：11

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