Feasibility of field-based light scattering spectroscopy

被引：24

作者：

Yang, CH ^{[1
]}

Perelman, LT ^{[1
]}

Wax, A ^{[1
]}

Dasari, RR ^{[1
]}

Feld, MS ^{[1
]}

机构：

[1] MIT, GR Harrison Spect Lab, Cambridge, MA 02139 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2000年 / 5卷 / 02期

关键词：

light scattering spectroscopy; interferometer; Mie theory;

D O I：

10.1117/1.429980

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible, A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 mu m in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed. (C) 2000 Society of Photo-Optical Instrumentation Engineers. [S1083-3668(00)00902-3].

引用

页码：138 / 143

页数：6

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