Inflammatory stress in primary venous and aortic endothelial cells of type 1 diabetic mice

被引:8
作者
Bucciarelli, Loredana G. [2 ]
Pollreisz, Andreas [1 ,4 ]
Kebschull, Moritz [4 ]
Ganda, Anjali [3 ]
Kalea, Anastasia Z. [1 ]
Hudson, Barry I. [1 ]
Zou, Yu Shan [1 ]
Lalla, Evanthia [4 ]
Ramasamy, Ravichandran [1 ]
Colombo, Paolo C. [3 ]
Schmidt, Ann Marie [1 ]
Yan, Shi Fang [1 ]
机构
[1] Columbia Univ, Med Ctr, Dept Surg, Div Surg Sci, New York, NY 10032 USA
[2] IRCCS, Ist Clin Humanitas, I-20089 Milan, Italy
[3] Columbia Univ, Med Ctr, Dept Med, New York, NY 10032 USA
[4] Columbia Univ, Med Ctr, Coll Dent Med, New York, NY 10032 USA
关键词
vein; artery; endothelium; inflammation; diabetes; OXIDATIVE STRESS; ACTIVATION; INHIBITION; APOPTOSIS; PROTEASE; HUMANS; DEATH;
D O I
10.1177/1479164109338775
中图分类号
R5 [内科学];
学科分类号
100201 [内科学];
摘要
Objective: The progression of diabetes is associated with profound endothelial dysfunction. We tested the hypothesis that cellular stress would be detectable in ECs retrieved from arterial and venous vessels of diabetic mice. Method: We describe a method for direct isolation of well-characterised aortic and venous ECs from mice in which cells are not subjected to propagation in culture. Results: Gene expression profiling, confirmed by real-time PCR, revealed a progressive increase in markers of injury within two main gene families, EC activation and EC apoptosis, in aortic and venous ECs recovered from diabetic versus non-diabetic mice. In short-term diabetes, IIIb mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. In long-term diabetes, casp-I mRNA transcripts were higher in aortic and venous ECs of diabetic mice versus controls. Conclusion: These data suggest that diabetes imparts diffuse endothelial perturbation in the arterial and venous endothelium.
引用
收藏
页码:249 / 261
页数:13
相关论文
共 32 条
[1]
Endothelial dysfunction and diabetes: roles of hyperglycemia, impaired insulin signaling and obesity [J].
Bakker, Wineke ;
Eringa, Etto C. ;
Sipkema, Pieter ;
van Hinsbergh, Victor W. M. .
CELL AND TISSUE RESEARCH, 2009, 335 (01) :165-189
[2]
Functional, cellular, and molecular characterization of the angiogenic response to chronic myocardial ischemia in diabetes [J].
Boodhwani, Munir ;
Sodha, Neel R. ;
Mieno, Shigetoshi ;
Xu, Shu-Hua ;
Feng, Jun ;
Ramlawi, Basel ;
Clements, Richard T. ;
Sellke, Frank W. .
CIRCULATION, 2007, 116 (11) :I31-I37
[3]
Deep vein thrombosis resolution is impaired in diet-induced type 2 diabetic mice [J].
Bouzeghrane, Fatiha ;
Zhang, Xiaochun ;
Gevry, Guylaine ;
Reymond, Jean .
JOURNAL OF VASCULAR SURGERY, 2008, 48 (06) :1575-1584
[4]
RAGE and modulation of ischemic injury in the diabetic myocardium [J].
Bucciarelli, Loredana G. ;
Ananthakrishnan, Radha ;
Hwang, Yuying C. ;
Kaneko, Michiyo ;
Soling, Fei ;
Sell, David R. ;
Strauch, Christopher ;
Monnier, Vincent M. ;
Yan, Shi Fang ;
Schmidt, Ann Marie ;
Ramasamy, Ravichandran .
DIABETES, 2008, 57 (07) :1941-1951
[5]
Hyperglycemia-induced reactive oxygen species toxicity to endothelial cells is dependent on paracrine mediators [J].
Busik, Julia V. ;
Mohr, Susanne ;
Grant, Maria B. .
DIABETES, 2008, 57 (07) :1952-1965
[6]
Hyperglycemia-induced apoptosis in mouse myocardium -: Mitochondrial cytochrome c-mediated caspase-3 activation pathway [J].
Cai, L ;
Li, W ;
Wang, GW ;
Guo, LP ;
Jiang, YC ;
Kang, YJ .
DIABETES, 2002, 51 (06) :1938-1948
[7]
Endothelial cell activation in patients with decompensated heart failure [J].
Colombo, PC ;
Banchs, JE ;
Celaj, S ;
Talreja, A ;
Lachmann, J ;
Malla, S ;
DuBois, NB ;
Ashton, AW ;
Latif, F ;
Jorde, UP ;
Ware, JA ;
LeJemtel, TH .
CIRCULATION, 2005, 111 (01) :58-62
[8]
Biopsy coupled to quantitative immunofluorescence: a new method to study the human vascular endothelium [J].
Colombo, PC ;
Ashton, AW ;
Celaj, S ;
Talreja, A ;
Banchs, JE ;
Dubois, NB ;
Marinaccio, M ;
Malla, S ;
Lachmann, J ;
Ware, JA ;
Le Jemtel, TH .
JOURNAL OF APPLIED PHYSIOLOGY, 2002, 92 (03) :1331-1338
[9]
Direct evidence of endothelial oxidative stress with aging in humans -: Relation to impaired endothelium-dependent dilation and upregulation of nuclear factor-κB [J].
Donato, Anthony J. ;
Eskurza, Iratxe ;
Silver, Annemarie E. ;
Levy, Adam S. ;
Pierce, Gary L. ;
Gates, Phillip E. ;
Seals, Douglas R. .
CIRCULATION RESEARCH, 2007, 100 (11) :1659-1666
[10]
Long-term stabilization of vein graft wall architecture and prolonged resistance to experimental atherosclerosis after E2F decoy oligonucleotide gene therapy [J].
Ehsan, A ;
Mann, MJ ;
Dell'Acqua, G ;
Dzau, VJ .
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 2001, 121 (04) :714-722