Transcriptional noise and the fidelity of initiation by RNA polymerase II

被引：527

作者：

Struhl, Kevin ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2007年 / 14卷 / 02期

关键词：

D O I：

10.1038/nsmb0207-103

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eukaryotes transcribe much of their genomes, but little is known about the fidelity of transcriptional initiation by RNA polymerase II in vivo. I suggest that similar to 90% of Pol II initiation events in yeast represent transcriptional noise, and that the specificity of initiation is comparable to that of DNA-binding proteins and other biological processes. This emphasizes the need to develop criteria that distinguish transcriptional noise from transcription with a biological function.

引用

页码：103 / 105

页数：3

共 25 条

[1] Nonsense-mediated mRNA decay: terminating erroneous gene expression [J].

Baker, KE ;

Parker, R .

CURRENT OPINION IN CELL BIOLOGY, 2004, 16 (03) :293-299

[2] Quantitation of the RNA polymerase II transcription machinery in yeast [J].

Borggrefe, T ;

Davis, R ;

Bareket-Samish, A ;

Kornberg, RD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (50) :47150-47153

[3] The transcriptional landscape of the mammalian genome [J].

Carninci, P ;

Kasukawa, T ;

Katayama, S ;

Gough, J ;

Frith, MC ;

Maeda, N ;

Oyama, R ;

Ravasi, T ;

Lenhard, B ;

Wells, C ;

Kodzius, R ;

Shimokawa, K ;

Bajic, VB ;

Brenner, SE ;

Batalov, S ;

Forrest, ARR ;

Zavolan, M ;

Davis, MJ ;

Wilming, LG ;

Aidinis, V ;

Allen, JE ;

Ambesi-Impiombato, X ;

Apweiler, R ;

Aturaliya, RN ;

Bailey, TL ;

Bansal, M ;

Baxter, L ;

Beisel, KW ;

Bersano, T ;

Bono, H ;

Chalk, AM ;

Chiu, KP ;

Choudhary, V ;

Christoffels, A ;

Clutterbuck, DR ;

Crowe, ML ;

Dalla, E ;

Dalrymple, BP ;

de Bono, B ;

Della Gatta, G ;

di Bernardo, D ;

Down, T ;

Engstrom, P ;

Fagiolini, M ;

Faulkner, G ;

Fletcher, CF ;

Fukushima, T ;

Furuno, M ;

Futaki, S ;

Gariboldi, M .

SCIENCE, 2005, 309 (5740) :1559-1563

[4] A high-resolution map of transcription in the yeast genome [J].

David, L ;

Huber, W ;

Granovskaia, M ;

Toedling, J ;

Palm, CJ ;

Bofkin, L ;

Jones, T ;

Davis, RW ;

Steinmetz, LM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (14) :5320-5325

[5] Tails of intrigue: Phosphorylation of RNA polymerase II mediates histone methylation [J].

Hampsey, M ;

Reinberg, D .

CELL, 2003, 113 (04) :429-432

[6] Dissecting the regulatory circuitry of a eukaryotic genome [J].

Holstege, FCP ;

Jennings, EG ;

Wyrick, JJ ;

Lee, TI ;

Hengartner, CJ ;

Green, MR ;

Golub, TR ;

Lander, ES ;

Young, RA .

CELL, 1998, 95 (05) :717-728

[7] Absolute mRNA levels and transcriptional initiation rates in Saccharomyces cerevisiae [J].

Iyer, V ;

Struhl, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (11) :5208-5212

[8] Evidence for distinct mechanisms facilitating transcript elongation through chromatin in vivo [J].

Kristjuhan, A ;

Svejstrup, JQ .

EMBO JOURNAL, 2004, 23 (21) :4243-4252

[9] Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme [J].

Kuras, L ;

Struhl, K .

NATURE, 1999, 399 (6736) :609-613

[10] RNA degradation by the exosome is promoted by a nuclear polyade nylation complex [J].

LaCava, J ;

Houseley, J ;

Saveanu, C ;

Petfalski, E ;

Thompson, E ;

Jacquier, A ;

Tollervey, D .

CELL, 2005, 121 (05) :713-724

← 1 2 3 →