Carbonic anhydrase inhibition prevents and reverts cardiomyocyte hypertrophy

被引:66
作者
Alvarez, Bernardo V.
Johnson, Danielle E.
Sowah, Daniel
Soliman, Daniel
Light, Peter E.
Xia, Ying
Karmazyn, Morris
Casey, Joseph R. [1 ]
机构
[1] Univ Alberta, Dept Physiol, Membrane Prot Res Grp, Edmonton, AB T6G 2H7, Canada
[2] Univ Alberta, Dept Pharmacol, Edmonton, AB T6G 2H7, Canada
[3] Univ Western Ontario, Dept Physiol & Pharmacol, London, ON N6A 5C1, Canada
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2007年 / 579卷 / 01期
关键词
D O I
10.1113/jphysiol.2006.123638
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Hypertrophic cardiomyocyte growth contributes substantially to the progression of heart failure. Activation of the plasma membrane Na+-H+ exchanger (NHE1) and Cl--HCO3- exchanger (AE3) has emerged as a central point in the hypertrophic cascade. Both NHE1 and AE3 bind carbonic anhydrase (CA), which activates their transport flux, by providing H+ and HCO3-, their respective transport substrates. We examined the contribution of CA activity to the hypertrophic response of cultured neonatal and adult rodent cardiomyocytes. Phenylephrine (PE) increased cell size by 37 +/- 2% and increased expression of the hypertrophic marker, atrial natriuretic factor mRNA, twofold in cultured neonatal rat cardiomyocytes. Cell size was also increased in adult cardiomyocytes subjected to angiotensin II or PE treatment. These effects were associated with increased expression of cytosolic CAII protein and the membrane-anchored isoform, CAIV. The membrane-permeant CA inhibitor, 6-ethoxyzolamide (ETZ), both prevented and reversed PE-induced hypertrophy in a concentration-dependent manner in neonate cardiomyocytes (IC50 = 18 mu M). ETZ and the related CA inhibitor methazolamide prevented hypertrophy in adult cardiomyocytes. In addition, ETZ inhibited transport activity of NHE1 and the AE isoform, AE3, with respective EC50 values of 1.2 +/- 0.3 mu M and 2.7 +/- 0.3 mu M. PE significantly increased neonatal cardiomyocyte Ca2+ transient frequency from 0.33 +/- 0.4 Hz to 0.77 +/- 0.04 Hz following 24 h treatment; these Ca2+-handling abnormalities were completely prevented by ETZ (0.28 +/- 0.07 Hz). Our study demonstrates a novel role for CA in mediating the hypertrophic response of cardiac myocytes to PE and suggests that CA inhibition represents an effective therapeutic approach towards mitigation of the hypertrophic phenotype.
引用
收藏
页码:127 / 145
页数:19
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