Bifunctional magnetic silica nanoparticles for highly efficient human stem cell labeling

被引:449
作者
Lu, Chen-Wen
Hung, Yann
Hsiao, Jong-Kai
Yao, Ming
Chung, Tsai-Hua
Lin, Yu-Shen
Wu, Si-Han
Hsu, Szu-Chun
Liu, Hon-Man
Mou, Chung-Yuan
Yang, Chung-Shi
Huang, Dong-Ming
Chen, Yao-Chang
机构
[1] Natl Hlth Res Inst, Ctr Nanomed Res, Miaoli 350, Taiwan
[2] Natl Hlth Res Inst, Stem Cell Res Ctr, Miaoli 350, Taiwan
[3] Natl Taiwan Univ, Dept Chem, Taipei 112, Taiwan
[4] Natl Taiwan Univ Hosp, Dept Lab Med, Taipei 112, Taiwan
[5] Natl Taiwan Univ Hosp, Dept Med Imaging, Taipei 112, Taiwan
[6] Coll Med, Taipei 112, Taiwan
关键词
D O I
10.1021/nl0624263
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A superparamagnetic iron oxide (SPIO) nanoparticle is emerging as an ideal probe for noninvasive cell tracking. However, its low intracellular labeling efficiency has limited the potential usage and has evoked great interest in developing new labeling strategies. We have developed fluorescein isothiocyanate (FITC)-incorporated silica-coated core-shell SPIO nanoparticles, SPIO@SiO2(FITC), with diameters of 50 nm, as a bifunctionally magnetic vector that can efficiently label human mesenchymal stem cells (hMSCs), via clathrin- and actin-dependent endocytosis with subsequent intracellular localization in late endosomes/lysosomes. The uptake process displays a time- and dose-dependent behavior. In our system, SPIO@SiO2(FITC) nanoparticles induce sufficient cell MRI contrast at an incubation dosage as low as 0.5 mu g of iron/mL of culture medium with 1.2 x 10(5) hMSCs, and the in vitro detection threshold of cell number is about 1 x 10(4) cells. Furthermore, 1.2 x 10(5) labeled cells can also be MRI-detected in a subcutaneous model in vivo. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes which can still be readily MRI detected. This is the first report that hMSCs can be efficiently labeled with MRI contrast nanoparticles and can be monitored in vitro and in vivo with a clinical 1.5-T MRI imager under low incubation concentration of iron oxide, short incubation time, and low detection cell numbers at the same time.
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收藏
页码:149 / 154
页数:6
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