Identification and characterization of (GA/CT)(n)-microsatellite loci from Quercus petraea

被引：291

作者：

Steinkellner, H

Fluch, S

Turetschek, E

Lexer, C

Streiff, R

Kremer, A

Burg, K

Glossl, J

机构：

[1] FORSCHUNGSZENTRUM SEIBERSDORF, A-2444 SEIBERSDORF, AUSTRIA

[2] INRA, STN RECH FORESTIERES BORDEAUX CESTAS, F-33610 CESTAS, FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1997年 / 33卷 / 06期

关键词：

genetic marker; simple sequence repeat; microsatellite; oak genome; Quercus;

D O I：

10.1023/A:1005736722794

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)(n)-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in e. petraea and Q. robur. 17 out Of 52 tested primer pairs resulted in the amplification of a polymorphic single-locus pattern. The number of alleles found per locus varied from 6 to 16. Combining the genetic variation observed for the characterized loci provides a unique genotype for all the individuals tested. Using intraspecific controlled crosses of e. robur trees Mendelian inheritance could be shown for five loci.

引用

页码：1093 / 1096

页数：4

共 24 条

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