Generation of active oxygen species from advanced glycation end-products (AGE) under ultraviolet light a (UVA) irradiation

To clarify a possible role of advanced glycation endproducts (AGE) on photoaging of human shin, the interaction between AGE and ultraviolet A light (UVA) was examined from both a biological and chemical perspective, Human dermal fibroblasts that were exposed to WA in the presence of AGE bound with bovine serum albumin (AGE-BSA) exhibited a significant de crease of cell viability as compared to control cells, which were exposed to WA in the absence of AGE-BSA, Further, WA-irradiated AGE-BSA reduced nitroblue tetrazolium to its formazan, but the reaction was inhibited by addition of superoxide dismutase in the system, WA dose dependent formation of H2O2 in AGE-BSA was also observed, An ESR spin-trapping study revealed the generation of unstable free radicals in AGE-BSA under WA irradiation. After addition of Fe2+ in the system, an ESR spectrum due to the formation of hydroxyl radicals was observed, On the basis of these results, the authors propose that AGE is an important factor for promoting photoaging in the skin Dia generation of active oxygen species involving . O-2(-), H2O2, and . OH. (C) 1997 Academic Press.