Simultaneous CO2- and O-16(2)/O-18(2)-gas exchange and fluorescence measurements indicate differences in light energy dissipation between the wild type and the phytochrome-deficient aurea mutant of tomato during water stress

被引:18
作者
Biehler, K
Haupt, S
Beckmann, J
Fock, H
Becker, TW
机构
[1] UNIV BIELEFELD,FAK BIOL,LEHRSTUHL GENET,D-33501 BIELEFELD,GERMANY
[2] UNIV KAISERSLAUTERN,FACHBEREICH BIOL,D-67653 KAISERSLAUTERN,GERMANY
关键词
water stress; gas exchange; fluorescence quenching; Lycopersicon esculentum; mutant; (tomato; aurea); energy dissipation;
D O I
10.1093/jxb/48.7.1439
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The CO2-, H2O- and O-16(2)/O-18(2) isotopic-gas exchange and the fluorescence quenching by attached leaves of the wild-type and of the phytochrome-deficient tomato aurea mutant was compared in relation to water stress and the photon fluence rate, The chlorophyll content of aurea leaves was reduced and the ultra-structure of the chloroplasts was altered, Nevertheless, the maximum rate of net CO2 uptake in air by the yellow-green leaves of the aurea mutant was similar to that by the dark-green wild-type leaves, However, less O-2 was produced by the leaves of the aurea mutant than by leaves of the wild-type, This result indicates a reduced rate of photosynthetic electron flux in aurea mutant leaves, No difference in both photochemical and nonphotochemical fluorescence quenching was found between wild-type and aurea mutant leaves, Water stress was correlated with a reversible decrease in the rates of both net CO2 uptake and transpiration hy wild-type and aurea mutant leaves, The rate of gross O-16(2) evolution by both wild-type and aurea mutant leaves was fairly unaffected by water stress, This result shows that in both wild-type and aurea leaves, the photochemical processes are highly resistant to water stress, The rate of gross O-18(2) uptake by wildtype leaves increased during water stress when the photon fluence rate was high, Under the same conditions, the rate of gross O-18(2) uptake by aurea mutant leaves remained unchanged. The physiological significane of this difference with respect to the (presumed) importance of oxygen reduction in photoprotection is discussed.
引用
收藏
页码:1439 / 1449
页数:11
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