Template boundary in a yeast telomerase specified by RNA structure

被引：112

作者：

Tzfati, Y ^{[1
]}

Fulton, TB ^{[1
]}

Roy, J ^{[1
]}

Blackburn, EH ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

来源：

SCIENCE | 2000年 / 288卷 / 5467期

关键词：

D O I：

10.1126/science.288.5467.863

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The telomerase ribonucleoprotein has a phylogenetically divergent RNA subunit, which contains a short template for telomeric DNA synthesis. To understand how telomerase RNA participates in mechanistic aspects of telomere synthesis, we studied a conserved secondary structure adjacent to the template. Disruption of this structure caused DNA synthesis to proceed beyond the normal template boundary, resulting in altered telomere sequences, telomere shortening, and cellular growth defects. Compensatory mutations restored normal telomerase function. Thus, the RNA structure, rather than its sequence, specifies the template boundary. This study reveals a specific function for an RNA structure in the enzymatic action of telomerase.

引用

页码：863 / 867

页数：5

共 26 条

[1]

AnsariLari MA, 1996, BIOTECHNIQUES, V21, P34

[2] BOUNDARY ELEMENTS OF THE TETRAHYMENA TELOMERASE RNA TEMPLATE AND ALIGNMENT DOMAINS [J].

AUTEXIER, C ;

GREIDER, CW .

GENES & DEVELOPMENT, 1995, 9 (18) :2227-2239

[3] Reconstitution of human telomerase activity and identification of a minimal functional region of the human telomerase RNA [J].

Autexier, C ;

Pruzan, R ;

Funk, WD ;

Greider, CW .

EMBO JOURNAL, 1996, 15 (21) :5928-5935

[4]

Blackburn E. H., 1999, RNA WORLD, P609

[5] Extension of life-span by introduction of telomerase into normal human cells [J].

Bodnar, AG ;

Ouellette, M ;

Frolkis, M ;

Holt, SE ;

Chiu, CP ;

Morin, GB ;

Harley, CB ;

Shay, JW ;

Lichtsteiner, S ;

Wright, WE .

SCIENCE, 1998, 279 (5349) :349-352

[6] Telomerase and the maintenance of chromosome ends [J].

Bryan, TM ;

Cech, TR .

CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (03) :318-324

[7] Identification of Kluyveromyces lactis telomerase:: Discontinuous synthesis along the 30-nucleotide-long templating domain [J].

Fulton, TB ;

Blackburn, EH .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (09) :4961-4970

[8] Flexible positioning of the telomerase-associated nuclease leads to preferential elimination of nontelomeric DNA [J].

Greene, EC ;

Bednenko, J ;

Shippen, DE .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (03) :1544-1552

[9] A TELOMERIC SEQUENCE IN THE RNA OF TETRAHYMENA TELOMERASE REQUIRED FOR TELOMERE REPEAT SYNTHESIS [J].

GREIDER, CW ;

BLACKBURN, EH .

NATURE, 1989, 337 (6205) :331-337

[10] Pausing of reverse transcriptase on retroviral RNA templates is influenced by secondary structures both 5′ and 3′ of the catalytic site [J].

Harrison, GP ;

Mayo, MS ;

Hunter, E ;

Lever, AML .

NUCLEIC ACIDS RESEARCH, 1998, 26 (14) :3433-3442

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