Metabolism of benzo[a]pyrene in fish hepatocytes cultured on microplates

被引：11

作者：

Ferraris, M ^{[1
]}

Dosanjh, MK ^{[1
]}

Marafante, E ^{[1
]}

机构：

[1] COMMISS EUROPEAN COMMUNITIES,JOINT RES CTR,INST ENVIRONM,EUROPEAN CTR VALIDAT ALTERNAT METHODS,I-21020 ISPRA,ITALY

来源：

POLYCYCLIC AROMATIC COMPOUNDS | 1996年 / 11卷 / 1-4期

关键词：

benzo[a]pyrene metabolism; fish hepatocytes; microplates; microassays; EROD PROD;

D O I：

10.1080/10406639608544653

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The metabolism of benzo[a]pyrene (BP) by trout and catfish hepatocytes seeded on 96-well microplates was examined. The 9,10-; 4,5-: and 7,8-dihydrodiols, 1,6-; 3,6- quinones and 3-OH BP were found to be the major metabolites in both the trout and catfish hepatocytes exposed to BP for 4 h. The results were compared with the corresponding long term culture of hepatocytes on porous membranes and in microsomes isolated from the whole liver. It was found that cells cultured on microplates retained their BP metabolizing activities for at least 6 d alter seeding. This method has the major advantage that only a small number of cells are required and in parallel, it is possible to perform cytotoxicity and cytogenetic microassays on cell populations characterized for their metabolic capacity.

引用

页码：91 / 98

页数：8

共 26 条

[1] REGULATION OF THE CYTOCHROME-P450 ENZYME-SYSTEM IN FISH [J].