Activation of crystalline cellulose to cellulose IIII results in efficient hydrolysis by cellobiohydrolase

被引：115

作者：

Igarashi, Kiyohiko ^{[1
]}

Wada, Masahisa ^{[1
]}

Samejima, Masahiro ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biomat Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

FEBS JOURNAL | 2007年 / 274卷 / 07期

关键词：

ammonia cellulose; cellobiohydrolase; cellobiose dehydrogenase; crystalline polymorphs; solid-liquid interface;

D O I：

10.1111/j.1742-4658.2007.05727.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystalline polymorphic form of cellulose (cellulose I-alpha-rich) of the green alga, Cladophora, was converted into cellulose IIII and I-beta by supercritical ammonium and hydrothermal treatments, respectively, and the hydrolytic rate and the adsorption of Trichoderma viride cellobiohydrolase I (Cel7A) on these products were evaluated by a novel analysis based on the surface density of the enzyme. Cellobiose production from cellulose IIII was more than 5 times higher than that from cellulose I. However, the amount of enzyme adsorbed on cellulose IIII was less than twice that on cellulose I, and the specific activity of the adsorbed enzyme for cellulose IIII was more than 3 times higher than that for cellulose I. When cellulose IIII was converted into cellulose I-beta by hydrothermal treatment, cellobiose production was dramatically decreased, although no significant change was observed in enzyme adsorption. This clearly indicates that the enhanced hydrolysis of cellulose IIII is related to the structure of the crystalline polymorph. Thus, supercritical ammonium treatment activates crystalline cellulose for hydrolysis by cellobiohydrolase.

引用

页码：1785 / 1792

页数：8

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