Study of the interaction of artemisinin with bovine serum albumin

被引:69
作者
Bian, Hedong [1 ]
Li, Mei [1 ]
Yu, Qing [1 ]
Chen, Zhenfeng [1 ]
Tian, Jianniao [1 ]
Liang, Hong [1 ]
机构
[1] Guangxi Normal Univ, Coll Chem & Chem Engn, Guangxi 541004, Peoples R China
基金
中国国家自然科学基金;
关键词
artemisinin; bovine serum albumin; binding; fluorescence; Fourier transform infrared spectroscopy; common ions; UVC irradiation;
D O I
10.1016/j.ijbiomac.2006.04.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The study on the interaction of artemisinin with bovine serum albumin (BSA) has been undertaken at three temperatures, 289, 296 and 303 K and investigated the effect of common ions and UVC (253.7 nm) irradiation on the binding of artemisinin with BSA. The binding mode, the binding constant and the protein structure changes in the presence of artemisinin in aqueous solution at pH 7.40 have been evaluated using fluorescence, UV-vis and Fourier transform infrared (FT-IR) spectroscopy. The quenching constant K-q, K-sv and the association constant K were calculated according to Stern-Volmer equation based on the quenching of the fluorescence of BSA. The thermodynamic parameters, the enthalpy (Delta H) and the entropy change (Delta S) were estimated to be -3.625 kJ mol(-1) and 107.419 J mol(-1) K-1 using the van't Hoff equation. The displacement experiment shows that artemisinin can bind to the subdomain IIA. The distance between the tryptophan residues in BSA and artemisinin bound to site I was estimated to be 2.22 nm using Foster's equation on the basis of fluorescence energy transfer. The decreased binding constant in the presence of enough common ions and UV C exposure, indicates that common ions and UV C irradiation have effect on artemisinin binding to BSA. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:291 / 297
页数:7
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