Hunting for the 5th base: Techniques for analyzing DNA methylation

被引：51

作者：

Ammerpohl, Ole ^{[1
]}

Martin-Subero, Jose I. ^{[1
]}

Richter, Julia ^{[1
]}

Vater, Inga ^{[1
]}

Siebert, Reiner ^{[1
]}

机构：

[1] Univ Kiel, Inst Human Genet, Univ Hosp Schleswig Holstein, D-24105 Kiel, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2009年 / 1790卷 / 09期

关键词：

DNA methylation; Maxam-Gilbert; Bisulphite sequencing; Methylation sensitive restriction enzyme; Methylated DNA immunoprecipitation; MeDIP; MSP; LUMA; PROMOTER METHYLATION; CPG-METHYLATION; METHYLTRANSFERASE INHIBITORS; CANCER; ASSAY; RESTRICTION; MECP2; 5-METHYLCYTOSINE; BINDING; GENE;

D O I：

10.1016/j.bbagen.2009.02.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: In the recent past large progress has been made in the analysis of the epigenome, the entirety of epigenetic modifications, and its meaning for the implementation of the genetic code. Besides histone modifications and miRNA expression, DNA methylation is one of the key players in the field of epigenetics, involved in numerous regulatory processes. Methods: In the present review we focus on methods for the analysis of DNA methylation patterns and present an overview about techniques and basic principles available for this purpose. Results and general significance: We here discuss advantages and disadvantages of various methods and their feasibility for specific tasks of DNA methylation analysis. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：847 / 862

页数：16

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