Competence regulation by oxygen in Streptococcus pneumoniae:: involvement of ciaRH and comCDE

被引：74

作者：

Echenique, JR ^{[1
]}

Chapuy-Regaud, S ^{[1
]}

Trombe, MC ^{[1
]}

机构：

[1] Univ Toulouse 3, Ctr Hosp Univ Rangueil, Lab Genet & Physiol Bacterienne, F-31403 Toulouse, France

来源：

MOLECULAR MICROBIOLOGY | 2000年 / 36卷 / 03期

关键词：

D O I：

10.1046/j.1365-2958.2000.01891.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anaerobic aerotolerant Streptococcus pneumoniae modulates its genetic transformability and its virulence in response to the oxygen concentration. The activity of a single protein encoded by nox and showing NADH oxidase activity is involved in these adaptive responses to O-2. Northern blot analysis of wild-type cultures grown under aerobic and microaerobic conditions indicated transcriptional control of comCDE by O-2. An O-2-independent mutant strain carrying the gain-of-function mutation comE38KE was isolated and its analysis showed that ComE is a key point in competence stimulation by O-2. Plasmid insertion mutations in ciaRH revealed that this two component signal-transducing system negatively regulates comCDE transcription. The level of comCDE transcripts appears as a major control point in competence regulation by O-2 and also by growth phase and cell density.

引用

页码：688 / 696

页数：9

共 32 条

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