Comparison of PCR and Culture for Screening of Vancomycin-Resistant Enterococci: Highly Disparate Results for vanA and vanB

被引:27
作者
Mak, Anton [3 ]
Miller, Mark A. [1 ]
Chong, George [2 ]
Monczak, Yury [2 ]
机构
[1] McGill Univ, Jewish Gen Hosp, Div Clin Microbiol, Dept Microbiol, Montreal, PQ H3T 1E2, Canada
[2] McGill Univ, Jewish Gen Hosp, Dept Pathol, Montreal, PQ H3T 1E2, Canada
[3] McGill Univ, Ctr Hlth, Dept Microbiol, Montreal, PQ, Canada
关键词
CARRIAGE; ASSAY; IDENTIFICATION; OUTBREAK; FAECIUM; FECES;
D O I
10.1128/JCM.01547-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We compared PCR to conventional culture for the detection of vancomycin-resistant enterococci (VRE) in 30,835 rectal samples over a 3-year period. The positive and negative predictive values of vanB PCR were 1.42% and 99.9%, respectively. A positive vanB result by PCR is poorly predictive and necessitates culture for differentiation of VRE-positive and -negative individuals.
引用
收藏
页码:4136 / 4137
页数:2
相关论文
共 14 条
[1]   Molecular characterization of vanB elements in naturally occurring gut anaerobes [J].
Ballard, SA ;
Pertile, KK ;
Lim, M ;
Johnson, PDR ;
Grayson, ML .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (05) :1688-1694
[2]   Comparison of three PCR primer sets for identification of vanB gene carriage in feces and correlation with carriage of vancomycin-resistant enterococci:: Interference by vanB-containing anaerobic bacilli [J].
Ballard, SA ;
Grabsch, EA ;
Johnson, PDR ;
Grayson, ML .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (01) :77-81
[3]  
CONDON S, 2008, 48 ANN INT C ANT AG, P227
[4]   Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces [J].
Domingo, MC ;
Huletsky, A ;
Bernal, A ;
Giroux, R ;
Boudreau, DK ;
Picard, FJ ;
Bergeron, MG .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 55 (04) :466-474
[5]   Vancomycin-resistant enterococci: Mechanisms and clinical observations [J].
Gold, HS .
CLINICAL INFECTIOUS DISEASES, 2001, 33 (02) :210-219
[6]   High rates of fecal carriage of nonenterococcal vanB in both children and adults [J].
Graham, M. ;
Ballard, S. A. ;
Grabsch, E. A. ;
Johnson, P. D. R. ;
Grayson, M. L. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (03) :1195-1197
[7]   Experience with the Roche LightCycler VRE detection kit during a large outbreak of vanB2/B3 vancomycin-resistant Enterococcus faecium [J].
Koh, T. H. ;
Deepak, R. N. ;
Se-Thoe, S. Y. ;
Lin, R. V. T. P. ;
Koay, E. S. C. .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 60 (01) :182-183
[8]   First report of vancomycin-resistant Streptococcus mitis bacteremia in a leukemic patient after prophylaxis with quinolones and during treatment with vancomycin [J].
Krcmery, V ;
Spanik, S ;
Trupl, J .
JOURNAL OF CHEMOTHERAPY, 1996, 8 (04) :325-326
[9]  
MORGAN MA, 2008, 48 ANN INT C ANT AG, P227
[10]   Convenient selective differential broth for isolation of vancomycin-resistant enterococcus from fecal material [J].
Novicki, TJ ;
Schapiro, JM ;
Ulness, BK ;
Sebeste, A ;
Busse-Johnston, L ;
Swanson, KM ;
Swanzy, SR ;
Leisenring, W ;
Limaye, AP .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) :1637-1640