New ratiometric fluorescent calcium indicators with moderately attenuated binding affinities

被引：21

作者：

Gee, KR ^{[1
]}

Archer, EA ^{[1
]}

Lapham, LA ^{[1
]}

Leonard, ME ^{[1
]}

Zhou, ZL ^{[1
]}

Bingham, J ^{[1
]}

Diwu, Z ^{[1
]}

机构：

[1] Mol Probes Inc, Eugene, OR 97402 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 14期

关键词：

D O I：

10.1016/S0960-894X(00)00280-8

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Mono-halogenated derivatives of the calcium indicators fura-2 and indo-1 were synthesized and their spectroscopic properties evaluated. Halogenation ortho or para to the bridging oxygen in the BAPTA nucleus had a more pronounced weakening effect on binding affinity than in the meta position in the fura derivatives. Two new excitation ratioable fluorescent calcium indicators, benzothiaza-1 and 2, were also synthesized. K-d values of 400 nM to 5.3 mu M [Ca2+] were observed in these families of new probes. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1515 / 1518

页数：4

共 14 条

[1] Spatially and functionally distinct Ca2+ stores in sarcoplasmic and endoplasmic reticulum [J].