In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium

被引:702
作者
Boisset, Jean-Charles [1 ,2 ]
van Cappellen, Wiggert [3 ]
Andrieu-Soler, Charlotte [1 ]
Galjart, Niels [1 ]
Dzierzak, Elaine [1 ,2 ]
Robin, Catherine [1 ,2 ]
机构
[1] Erasmus MC, Dept Cell Biol, NL-3000 CA Rotterdam, Netherlands
[2] Erasmus MC, Erasmus Stem Cell Inst, NL-3000 CA Rotterdam, Netherlands
[3] Erasmus MC, Dept Reprod & Dev, NL-3000 CA Rotterdam, Netherlands
关键词
STEM-CELLS; AGM REGION; DEFINITIVE HEMATOPOIESIS; HAEMOGENIC ENDOTHELIUM; EMBRYO; EXPRESSION; GENERATION; MARKS; COLONIZATION; MIGRATION;
D O I
10.1038/nature08764
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Haematopoietic stem cells (HSCs), responsible for blood production in the adult mouse, are first detected in the dorsal aorta starting at embryonic day 10.5 (E10.5)(1-3). Immunohistological analysis of fixed embryo sections has revealed the presence of haematopoietic cell clusters attached to the aortic endothelium where HSCs might localize(4-6). The origin of HSCs has long been controversial and several candidates of the direct HSC precursors have been proposed (for review see ref. 7), including a specialized endothelial cell population with a haemogenic potential. Such cells have been described both in vitro in the embryonic stem cell (ESC) culture system(8,9) and retrospectively in vivo by endothelial lineage tracing(5,10) and conditional deletion experiments(11). Whether the transition from haemogenic endothelium to HSC actually occurs in the mouse embryonic aorta is still unclear and requires direct and real-time in vivo observation. To address this issue we used time-lapse confocal imaging and a new dissection procedure to visualize the deeply located aorta. Here we show the dynamic de novo emergence of phenotypically defined HSCs (Sca1(+), c-kit(+), CD41(+)) directly from ventral aortic haemogenic endothelial cells.
引用
收藏
页码:116 / U131
页数:6
相关论文
共 30 条
[1]   Biomechanical forces promote embryonic haematopoiesis [J].
Adamo, Luigi ;
Naveiras, Olaia ;
Wenzel, Pamela L. ;
McKinney-Freeman, Shannon ;
Mack, Peter J. ;
Gracia-Sancho, Jorge ;
Suchy-Dicey, Astrid ;
Yoshimoto, Momoko ;
Lensch, M. William ;
Yoder, Mervin C. ;
Garcia-Cardena, Guillermo ;
Daley, George Q. .
NATURE, 2009, 459 (7250) :1131-U120
[2]   CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis [J].
Bertrand, Julien Y. ;
Kim, Albert D. ;
Teng, Shutian ;
Traver, David .
DEVELOPMENT, 2008, 135 (10) :1853-1862
[3]   Haploinsufficiency of AML1 affects the temporal and spatial generation of hematopoietic stem cells in the mouse embryo [J].
Cai, ZL ;
de Bruijn, M ;
Ma, XQ ;
Dortland, B ;
Luteijn, T ;
Downing, JR ;
Dzierzak, E .
IMMUNITY, 2000, 13 (04) :423-431
[4]   Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter [J].
Chen, Michael J. ;
Yokomizo, Tomomasa ;
Zeigler, Brandon M. ;
Dzierzak, Elaine ;
Speck, Nancy A. .
NATURE, 2009, 457 (7231) :887-891
[5]   αIIb integrin expression during development of the murine hemopoietic system [J].
Corbel, C ;
Salaün, J .
DEVELOPMENTAL BIOLOGY, 2002, 243 (02) :301-311
[6]   Definitive hematopoietic stem cells first develop within the major arterial regions of the mouse embryo [J].
de Bruijn, MFTR ;
Speck, NA ;
Peeters, MCE ;
Dzierzak, E .
EMBO JOURNAL, 2000, 19 (11) :2465-2474
[7]   Hematopoietic stem cells localize to the endothelial cell layer in the midgestation mouse aorta [J].
de Bruijn, MFTR ;
Ma, XQ ;
Robin, C ;
Ottersbach, K ;
Sanchez, MJ ;
Dzierzak, E .
IMMUNITY, 2002, 16 (05) :673-683
[8]   Of lineage and legacy: the development of mammalian hematopoietic stem cells [J].
Dzierzak, Elaine ;
Speck, Nancy A. .
NATURE IMMUNOLOGY, 2008, 9 (02) :129-136
[9]   Continuous single-cell imaging of blood generation from haemogenic endothelium [J].
Eilken, Hanna M. ;
Nishikawa, Shin-Ichi ;
Schroeder, Timm .
NATURE, 2009, 457 (7231) :896-900
[10]  
GARCIAPORRERO JA, 1995, ANAT EMBRYOL, V192, P427