Self-Enhanced Accumulation of FtsN at Division Sites and Roles for Other Proteins with a SPOR Domain (DamX, DedD, and RlpA) in Escherichia coli Cell Constriction

被引:154
作者
Gerding, Matthew A. [1 ]
Liu, Bing [1 ]
Bendezu, Felipe O. [1 ]
Hale, Cynthia A. [1 ]
Bernhardt, Thomas G. [1 ]
de Boer, Piet A. J. [1 ]
机构
[1] Case Western Reserve Univ, Sch Med, Dept Mol Biol & Microbiol, Cleveland, OH 44106 USA
关键词
GREEN FLUORESCENT PROTEIN; L-ALANINE AMIDASES; SEPTAL RING; BACILLUS-SUBTILIS; GENE; RECRUITMENT; MACHINERY; MUTANTS; LOCALIZATION; SPORULATION;
D O I
10.1128/JB.00811-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Of the known essential division proteins in Escherichia coli, FtsN is the last to join the septal ring organelle. FtsN is a bitopic membrane protein with a small cytoplasmic portion and a large periplasmic one. The latter is thought to form an alpha-helical juxtamembrane region, an unstructured linker, and a C-terminal, globular, murein-binding SPOR domain. We found that the essential function of FtsN is accomplished by a surprisingly small essential domain ((E)FtsN) of at most 35 residues that is centered about helix H2 in the periplasm. (E)FtsN contributed little, if any, to the accumulation of FtsN at constriction sites. However, the isolated SPOR domain ((S)FtsN) localized sharply to these sites, while SPOR-less FtsN derivatives localized poorly. Interestingly, localization of (S)FtsN depended on the ability of cells to constrict and, thus, on the activity of (E)FtsN. This and other results suggest that, compatible with a triggering function, FtsN joins the division apparatus in a self-enhancing fashion at the time of constriction initiation and that its SPOR domain specifically recognizes some form of septal murein that is only transiently available during the constriction process. SPOR domains are widely distributed in bacteria. The isolated SPOR domains of three additional E. coli proteins of unknown function, DamX, DedD, and RlpA, as well as that of Bacillus subtilis CwlC, also accumulated sharply at constriction sites in E. coli, suggesting that septal targeting is a common property of SPORs. Further analyses showed that DamX and, especially, DedD are genuine division proteins that contribute significantly to the cell constriction process.
引用
收藏
页码:7383 / 7401
页数:19
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