Treatment of dystrophic epidermolysis bullosa with autologous meshed split-thickness skin grafts and allogeneic cultured keratinocytes

A 5-year-old boy with dystrophic epidermolysis bullosa (DEB), type generalized mitis, presented with a 2-year-old non-healing skin defect on the presternal and abdominal region. After debridement up to the middle dermis, the defect was grafted with autologuus meshed split thickness grafts from non-involved shin from the thigh and covered with allogeneic keratinocytes. The described method is a valuable therapeutic option in DEB patients who have donor skin areas with a preserved structure of the basement membrane zone.