Proteome Organization in a Genome-Reduced Bacterium

被引：357

作者：

Kuehner, Sebastian ^{[1
]}

van Noort, Vera ^{[1
]}

Betts, Matthew J. ^{[1
]}

Leo-Macias, Alejandra ^{[1
]}

Batisse, Claire ^{[1
]}

Rode, Michaela ^{[1
]}

Yamada, Takuji ^{[1
]}

Maier, Tobias ^{[2
]}

Bader, Samuel ^{[1
]}

Beltran-Alvarez, Pedro ^{[1
]}

Castano-Diez, Daniel ^{[1
]}

Chen, Wei-Hua ^{[1
]}

Devos, Damien ^{[1
]}

Gueell, Marc ^{[2
]}

Norambuena, Tomas ^{[3
]}

Racke, Ines ^{[1
]}

Rybin, Vladimir ^{[1
]}

Schmidt, Alexander ^{[4
]}

Yus, Eva ^{[2
]}

Aebersold, Ruedi ^{[4
]}

Herrmann, Richard ^{[5
]}

Boettcher, Bettina ^{[1
]}

Frangakis, Achilleas S. ^{[1
]}

Russell, Robert B. ^{[1
]}

Serrano, Luis ^{[2
,6
]}

Bork, Peer ^{[1
]}

Gavin, Anne-Claude ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

[2] Univ Pompeu Fabra, Ctr Regulac Genom, Barcelona 08003, Spain

[3] Pontificia Univ Catolica Chile, Santiago, Chile

[4] ETH, CH-8093 Zurich, Switzerland

[5] Univ Heidelberg, Zentrum Mol Biol, D-69120 Heidelberg, Germany

[6] ICREA, Barcelona 08010, Spain

来源：

SCIENCE | 2009年 / 326卷 / 5957期

基金：

欧洲研究理事会;

关键词：

TRANSFER-RNA SYNTHETASES; MYCOPLASMA-PNEUMONIAE; ESCHERICHIA-COLI; INTERACTION MAP; HELICOBACTER-PYLORI; COMPLEX-FORMATION; CELL-CYCLE; PROTEINS; SEQUENCE; DNA;

D O I：

10.1126/science.1176343

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The genome of Mycoplasma pneumoniae is among the smallest found in self-replicating organisms. To study the basic principles of bacterial proteome organization, we used tandem affinity purification-mass spectrometry (TAP-MS) in a proteome-wide screen. The analysis revealed 62 homomultimeric and 116 heteromultimeric soluble protein complexes, of which the majority are novel. About a third of the heteromultimeric complexes show higher levels of proteome organization, including assembly into larger, multiprotein complex entities, suggesting sequential steps in biological processes, and extensive sharing of components, implying protein multifunctionality. Incorporation of structural models for 484 proteins, single-particle electron microscopy, and cellular electron tomograms provided supporting structural details for this proteome organization. The data set provides a blueprint of the minimal cellular machinery required for life.

引用

页码：1235 / 1240

页数：6

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