Combined PI-DAPI staining (CPD) reveals NOR asymmetry and facilitates karyotyping of plant chromosomes

被引：17

作者：

Andras, SC

Hartman, TPV

Alexander, J

McBride, R

Marshall, JA

Power, JB

Cocking, EC

Davey, MR ^{[1
]}

机构：

[1] Univ Nottingham, Sch Biol Sci, Plant Sci Div, Nottingham NG7 2RD, England

[2] Univ Babes Bolyai, Fac Biol Geol, Dept Ecol & Genet, R-3400 Cluj Napoca, Romania

[3] Univ London Queen Mary & Westfield Coll, Sch Biol Sci, London E1 4NS, England

[4] Appl Imaging Int Ltd, Biosci Ctr, Newcastle Upon Tyne NE1 4EP, Tyne & Wear, England

来源：

CHROMOSOME RESEARCH | 2000年 / 8卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

chromosome banding; combined PI and DAPI (CPD); 4 ' 6-diamidino-2-phenylindol (DAPI); fluorescent staining; Lycopersicon; marker chromosome; nucleolar organiser region (NOR); Oryza; propidium iodide (PI);

D O I：

10.1023/A:1009258719052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper presents a preparative and staining procedure for plant mitotic chromosomes that uses a combination of PI (propidium iodide) and DAPI (4',6-diamidino-2-phenylindol) and which reveals a pattern of high-affinity regions for these fluorochromes. Nucleolar organiser regions (NORs), telomeres and centromeric regions exhibit high PI affinity (red), whereas other chromosomal regions exhibit high affinity for either PI (red) or DAPI (blue). NOR-bearing and other chromosomes are readily distinguished, facilitating karyotyping. The dual staining pattern was observed in all the plants tested. Aspects of NOR size, number and occurrence are discussed. A karyotype of rice metaphase chromosomes is presented, based on their fluorescent banding patterns.

引用

页码：387 / 391

页数：5

共 22 条

[1] A drop-spreading technique to produce cytoplasm-free mitotic preparations from plants with small chromosomes [J].