Naringin Protects Against Cartilage Destruction in Osteoarthritis Through Repression of NF-κB Signaling Pathway

被引：65

作者：

Zhao, Yunpeng ^{[1
]}

Li, Zhong ^{[2
]}

Wang, Wenhan ^{[3
]}

Zhang, Hui ^{[3
]}

Chen, Jianying ^{[4
]}

Su, Peng ^{[3
]}

Liu, Long ^{[3
]}

Li, Weiwei ^{[3
]}

机构：

[1] Shandong Univ, Qilu Hosp, Dept Orthopaed, 107 Wenhuaxi Rd, Jinan 250012, Shandong, Peoples R China

[2] Taishan Med Univ, Liaocheng Clin Acad, Dept Orthopaed, Liaocheng 252000, Shandong, Peoples R China

[3] Shandong Univ, Qilu Hosp, Dept Pathol, 107 Wenhuaxi Rd, Jinan 250012, Shandong, Peoples R China

[4] Inst Biopharmaceut Shandong Prov, Jinan 250101, Shandong, Peoples R China

来源：

INFLAMMATION | 2016年 / 39卷 / 01期

关键词：

TNF-alpha; osteoarthritis; chondrocyte; naringin; NF-kappa B signaling; INDUCED OXIDATIVE STRESS; MESENCHYMAL STEM-CELLS; TNF-ALPHA; INFLAMMATORY RESPONSE; ARTHRITIS; MICE; CHONDROCYTES; DEGRADATION; INHIBITION; EXPRESSION;

D O I：

10.1007/s10753-015-0260-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Naringin was previously reported as a multifunctional agent. Recently, naringin was found to play a protective role in various inflammatory conditions. However, the role of naringin in cartilage degeneration and osteoarthritis (OA) progression is still unknown. TNF-alpha is reported to play a detrimental role in OA. Herein, primary murine chondrocytes were isolated and cultured with stimulation of TNF-alpha, in the presence or absence of naringin treatment. As a result, naringin attenuated TNF-alpha-mediated inflammation and catabolism in chondrocyte. Besides, surgically induced OA mice models were established. Cartilage degradation and OA severity were evaluated using Safranin-O staining, immunohistochemistry, and ELISA. Moreover, levels of inflammatory cytokines and catabolic markers in OA were analyzed. Oral administration of naringin alleviated degradation of cartilage matrix and protected against OA development in the surgically induced OA models. Furthermore, the protective function of naringin in cartilage and chondrocyte was possibly due to suppression of NF-kappa B signaling pathway. Collectively, this study presents naringin as a potential target for the treatment of joint degenerative diseases, including OA.

引用

页码：385 / 392

页数：8

共 28 条

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In Vivo Luminescence Imaging of NF-κB Activity and Serum Cytokine Levels Predict Pain Sensitivities in a Rodent Model of Osteoarthritis [J].