Dimerization and DNA-binding of ASR1, a small hydrophilic protein abundant in plant tissues suffering from water loss

被引：49

作者：

Maskin, Laura

Frankel, Nicolas

Gudesblat, Gustavo

Demergasso, Maria J.

Pietrasanta, Lia I.

Iusem, Norberto D. ^{[1
]}

机构：

[1] Univ Buenos Aires, IFIBYNE CONICET, Fac Ciencias Exactas & Nat, Lab Fisiol & Biol Mol, RA-1428 Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Ctr Microscopias Avanzadas, RA-1428 Buenos Aires, DF, Argentina

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 352卷 / 04期

关键词：

Asr genes; Lycopersicon esculentum; seed; dimerization; atomic force microscopy; GENE; EXPRESSION; LOCALIZATION; DROUGHT; DEFICIT; SUGAR; ABA;

D O I：

10.1016/j.bbrc.2006.11.115

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Asr gene family is present in Spermatophyta. Its members are generally activated under water stress. We present evidence that tomato ASR1, one of the proteins of the family, accumulates in seed during late stages of embryogenesis, a physiological process characterized by water loss. In vitro, electrophoretic assays show a homo-dimeric structure for ASR1 and highlight strong non-covalent interactions between monomers prone to self-assemble. Direct visualization of single molecules by atomic force microscopy (AFM) confirms that ASR1 forms homodimers and that uncovers both monomers and dimers bind double stranded DNA. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：831 / 835

页数：5

共 27 条

[1] Reduction/oxidation-phosphorylation control of DNA binding in the bZIP dimerization network [J].

Amoutzias, Gregory D. ;

Bornberg-Bauer, Erich ;

Oliver, Stephen G. ;

Robertson, David L. .

BMC GENOMICS, 2006, 7 (1)

[2]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[3] ATOMIC FORCE MICROSCOPE [J].

BINNIG, G ;

QUATE, CF ;

GERBER, C .

PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933

[4] Oligomeric protein structure networks: insights into protein-protein interactions [J].

Brinda, KV ;

Vishveshwara, S .

BMC BIOINFORMATICS, 2005, 6 (1)

[5]

Bustamante C, 1996, ANNU REV BIOPH BIOM, V25, P395

[6] A grape ASR protein involved in sugar and abscisic acid signaling [J].

Çakir, B ;

Agasse, A ;

Gaillard, C ;

Saumonneau, A ;

Delrot, S ;

Atanassova, R .

PLANT CELL, 2003, 15 (09) :2165-2180

[7] Heard it through the grapevine? ABA and sugar cross-talk: the ASR story [J].

Carrari, F ;

Fernie, AR ;

Iusem, ND .

TRENDS IN PLANT SCIENCE, 2004, 9 (02) :57-59

[8] PURIFICATION OF A MAIZE DEHYDRIN [J].

CECCARDI, TL ;

MEYER, NC ;

CLOSE, TJ .

PROTEIN EXPRESSION AND PURIFICATION, 1994, 5 (03) :266-269

[9] Abscisic acid-inducible nuclear proteins bind to bipartite promoter elements required for ABA response and embryo-regulated expression of the carrot Dc3 gene [J].

Chung, HJ ;

Fu, HY ;

Thomas, TL .

PLANTA, 2005, 220 (03) :424-433

[10]

Demir I., 1992, Seed Science Research, V2, P81, DOI 10.1017/S0960258500001173

← 1 2 3 →