Characterization of polymorphisms in the toxin A and B genes of Clostridium difficile

被引：78

作者：

Rupnik, M

Braun, V

Soehn, F

Janc, M

Hofstetter, M

LaufenbergFeldmann, R

vonEichelStreiber, C

机构：

[1] UNIV MAINZ,VERFUGUNGSGEBAUDE FORSCH & ENTWICKLUNG,INST MED MIKROBIOL & HYG,D-55101 MAINZ,GERMANY

[2] UNIV LJUBLJANA,DEPT BIOL,LJUBLJANA 61000,SLOVENIA

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 148卷 / 02期

关键词：

polymerase chain reaction; Clostridium difficile; genetic polymorphism; toxin A; toxin B;

D O I：

10.1016/S0378-1097(97)00033-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have used six independent polymerase chain reactions (A1-A3 and B1-B3) for amplification of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all over the genes, and (2) deletions that could be found only in tcdA. Characteristic differences between strains were mainly focused to the 5' third of tcdB (B1 fragment) and/or the 3' third of tcdA (A3 fragment). The possible use of our approach for typing of C. difficile toxin genes is discussed.

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页码：197 / 202

页数：6

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