Bio-LDH nanohybrid for gene therapy

被引：152

作者：

Kwak, SY

Jeong, YJ

Park, JS

Choy, JH ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Mol Engn, Natl Nanohybrid Mat Lab, Seoul 151747, South Korea

[2] Seoul Natl Univ, Sch Chem & Mol Engn, Genet Biochem Lab, Seoul 151747, South Korea

来源：

SOLID STATE IONICS | 2002年 / 151卷 / 1-4期

关键词：

bioinorganic nanohybrid; anionic clays; layered compounds; nonviral vector;

D O I：

10.1016/S0167-2738(02)00714-2

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Nano-sized inorganic clay, such as layered double hydroxide (LDH), has been demonstrated as delivery carrier for genes and drugs by hybridizing with DNA and c-antisense oligonucleotide (As-myc). Upon intercalating biomolecules into hydroxide layers, the basal spacing of LDH increases from 8.7 Angstrom (for NO3-) to 23.9 Angstrom (DNA) and 17.1 Angstrom (As-myc), respectively. A strong suppression of cell growth (65%) is observed when the HL-60 cells are incubated with 20 muM As-myc-LDH hybrid. However, LDH itself is found to be noncytotoxic on HL-60 cells (leukemia cells). Based on these findings, it is proved that LDHs can act as a new inorganic carrier which is completely different from ever existing nonviral vectors in terms of its chemical bonding and structure. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：229 / 234

页数：6

共 40 条

[1]

BENNETT CF, 1992, MOL PHARMACOL, V41, P1023

[2] Layer-anion interactions in magnesium aluminum layered double hydroxides intercalated with cobalticyanide and nitroprusside [J].