RNA editing by ADARs is important for normal behavior in Caenorhabditis elegans

被引:175
作者
Tonkin, LA
Saccomanno, L
Morse, DP
Brodigan, T
Krause, M
Bass, BL
机构
[1] Univ Utah, Dept Biochem, Salt Lake City, UT 84132 USA
[2] Univ Utah, Howard Hughes Med Inst, Salt Lake City, UT 84132 USA
[3] NIDDKD, Mol Biol Lab, NIH, Bethesda, MD 20892 USA
关键词
chemotaxis; double-stranded RNA; inosine; neuronal; vulva;
D O I
10.1093/emboj/cdf607
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Here we take advantage of the well-characterized and simple nervous system of Caenorhabditis elegans to further our understanding of the functions of RNA editing. We describe the two C.elegans ADAR genes, adr-1 and adr-2, and characterize strains containing homozygous deletions in each, or both, of these genes. We find that adr-1 is expressed in most, if not all, cells of the C.elegans nervous system and also in the developing vulva. Using chemotaxis assays, we show that both ADARs are important for normal behavior. Biochemical, molecular and phenotypic analyses indicate that ADR-1 and ADR-2 have distinct roles in C.elegans, but sometimes act together.
引用
收藏
页码:6025 / 6035
页数:11
相关论文
共 43 条
[1]  
Anderson P, 1995, METHOD CELL BIOL, V48, P31
[2]   Our genome unveiled [J].
Baltimore, D .
NATURE, 2001, 409 (6822) :814-816
[3]   Signal transduction in the Caenorhabditis elegans nervous system. [J].
Bargmann, CI ;
Kaplan, JM .
ANNUAL REVIEW OF NEUROSCIENCE, 1998, 21 :279-308
[4]   ODORANT-SELECTIVE GENES AND NEURONS MEDIATE OLFACTION IN C-ELEGANS [J].
BARGMANN, CI ;
HARTWIEG, E ;
HORVITZ, HR .
CELL, 1993, 74 (03) :515-527
[5]   RNA editing by adenosine deaminases that act on RNA [J].
Bass, BL .
ANNUAL REVIEW OF BIOCHEMISTRY, 2002, 71 :817-846
[6]   A DEVELOPMENTALLY REGULATED ACTIVITY THAT UNWINDS RNA DUPLEXES [J].
BASS, BL ;
WEINTRAUB, H .
CELL, 1987, 48 (04) :607-613
[7]   Protein diversity from alternative splicing: A challenge for bioinformatics and post-genome biology [J].
Black, DL .
CELL, 2000, 103 (03) :367-370
[8]   Regulation of serotonin-2C receptor G-protein coupling by RNA editing [J].
Burns, CM ;
Chu, H ;
Rueter, SM ;
Hutchinson, LK ;
Canton, H ;
SandersBush, E ;
Emeson, RB .
NATURE, 1997, 387 (6630) :303-308
[9]   Meiotic recombination in C-elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis [J].
Dernburg, AF ;
McDonald, K ;
Moulder, G ;
Barstead, R ;
Dresser, M ;
Villeneuve, AM .
CELL, 1998, 94 (03) :387-398
[10]  
Eisenmann DM, 2000, GENETICS, V156, P1097