Intermediate chains of exopolysaccharides from Lactobacillus rhamnosus RW-9595M increase IL-10 production by macrophages

被引：164

作者：

Bleau, C. ^{[1
]}

Monges, A. ^{[1
]}

Rashidan, K. ^{[1
]}

Laverdure, J. -P. ^{[1
]}

Lacroix, M. ^{[2
]}

Van Calsteren, M. -R. ^{[3
]}

Millette, M. ^{[2
]}

Savard, R. ^{[1
]}

Lamontagne, L. ^{[1
]}

机构：

[1] Univ Quebec, Dept Sci Biol, Montreal, PQ H3C 3P8, Canada

[2] INRS Inst Armand Frappier, Res Lab Sci Appl Food, Laval, PQ, Canada

[3] Agr & Agroalimentaire Canada, Ctr Rech & Dev Aliments, St Hyacinthe, PQ, Canada

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2010年 / 108卷 / 02期

关键词：

cytokines; exopolysaccharides; Lactobacillus; lymphocytes; macrophages; CLOSTRIDIUM-DIFFICILE COLITIS; TNF-ALPHA PRODUCTION; LIPOTEICHOIC ACID; IFN-GAMMA; T-CELLS; INTERLEUKIN-10; PROBIOTICS; STRAINS;

D O I：

10.1111/j.1365-2672.2009.04450.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Aims: To evaluate the immunosuppressive properties of the exopolysaccharide (EPS) from high-EPS producer Lactobacillus rhamnosus RW-9595M on inflammatory cytokines produced by macrophages. Methods and Results: The conditioned media (CM) were produced by macrophages treated with parental Lact. rhamnosus ATCC 9595 and its isogenic variant, the high-EPS producer Lact. rhamnosus RW-9595M, and the levels of TNF-alpha, IL-6, IL-10 and IL-12 were evaluated. Results revealed that CM from parental Lact. rhamnosus induced higher levels of TNF-alpha, IL-6 and IL-12 but inhibited IL-10 production, whereas its mucous variant induced low or no TNF-alpha and IL-6. Addition of purified EPS to macrophages treated with parental Lact. rhamnosus decreased the inflammatory cytokines and inhibited the metabolic activity of lymphocytes. The intermediate polysaccharide chains (16-30 units) produced by time-controlled hydrolysis of EPS increased the IL-10 produced by macrophages. Conclusions: Polysaccharide chains of EPS induced immunosuppression by the production of macrophagic anti-inflammatory IL-10. Significance and impact of the Study: These results indicate that the EPS from Lact. rhamnosus RW-9595M may be useful as a new immunosuppressive product in dairy food.

引用

页码：666 / 675

页数：10

共 32 条

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