Establishment and characterization of a unique 1 μm diameter liver-derived progenitor cell line

被引：6

作者：

Aravalli, Rajagopal N. ^{[1
]}

Sahin, M. Behnan ^{[2
]}

Cressman, Erik N. K. ^{[1
]}

Steer, Clifford J. ^{[2
,3
]}

机构：

[1] Univ Minnesota, Sch Med, Dept Radiol, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Sch Med, Dept Med, Minneapolis, MN 55455 USA

[3] Univ Minnesota, Sch Med, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 391卷 / 01期

关键词：

Stem cell; Progenitor; Liver; Cell line; Cancer; HEMATOPOIETIC STEM-CELLS; SIZE CONTROL; G(1) CONTROL; IN-VITRO; IDENTIFICATION; DIFFERENTIATE; HEPATOCYTES; IMMORTALIZATION; REGENERATION; ETHIONINE;

D O I：

10.1016/j.bbrc.2009.11.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Liver-derived progenitor cells (LDPCs) are recently identified novel stem/progenitor cells from healthy, unmanipulated adult rat livers. They are distinct from other known liver stem/progenitor cells such as the oval cells in this study, we have generated a LDPC cell line RA1 by overexpressing the simian virus 40 (SV40) large T antigen (TAg) in primary LDPCs. This cell line was propagated continuously for 55 passages in culture, after which it became senescent. Interestingly, following transformation with SV40 TAg, LDPCs decreased in size significantly and the propagating cells measured 1 mu m in diameter RA1 cells proliferated in vitro with a doubling time of 5-7 days, and expressed cell surface markers of LDPCs. in this report, we describe the characterization of this novel progenitor cell line that might serve as a valuable model to study liver cell functions and stem cell origin of liver cancers (C) 2009 Elsevier Inc All rights reserved.

引用

页码：56 / 62

页数：7

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