The distributions of C-3 and C-4 grasses were used to interpret the distribution, seasonal performance, and potential production of grasslands in the Great Plains of North America. Thirteen major grassland seasonal land cover classes were studied with data from three distinct sources. Normalized Difference Vegetation Index (NDVI) data derived from the National Oceanic and Atmospheric Administration (NOAA) Advanced Very High Resolution Radiometer (AVHRR) sensor were collected for each pixel over a 5-yr period (1989-1993), analyzed for quantitative attributes and seasonal relationships, and then aggregated by land cover class. Data from the State Soil Geographic (STATSGO) database were used to identify dominant plant species contributing to the potential production in each map unit. These species were identified as C-3 or C-4, and contributions to production were aggregated to provide estimates of the percentage of C-3 and C-4 production for each intersection of the STATSGO map units and the seasonal land cover classes. Carbon isotope values were obtained at specific sites from the soil organic matter of the upper horizon of soil cores and were related to STATSGO estimates of potential production. The grassland classes were distributed with broad northwest-to-southeast orientations. Some classes had large variations in C-3 and C-4 composition with high proportions of C-4 species in the south and low proportions in the north. This diversity of photosynthetic types within land cover classes that cross regions of different temperature and precipitation results in similar seasonal patterns and magnitudes of NDVI. The easternmost class, 65, containing tallgrass prairie components, bluestem, Indiangrass, and switchgrass, possessed the highest maximum NDVI and time-integrated NDVI values each year. Grassland classes varied over 5 yr from a high integrated NDVI mean of 4.9 in class 65 in the east to a low of 1.2 in class 76 (sand sage, blue grama, wheatgrass, and buffalograss) in the southwest. Although environmental conditions varied widely during the 5 yr, the rankings of class performance were consistent across years for these NDVI metrics. Land cover classes were less consistent in time of onset, which was often earlier in areas in the north dominated by C-4 grasses than in areas to the south dominated by C-4 grasses. At the level of seasonal land cover classes, no significant relationship was found between the proportions of C-3 and C-4 species and estimates of potential production derived from the STATSGO database or inferred from the seasonal patterns of NDVI. The isotopic data from specific sites and the potential production data from STATSGO suggest similar patterns of high proportional production by C-4 species throughout the south and a decline in proportional production north of the central Great Plains. The land cover classes integrate ecosystem units that encompass a wide diversity of species and C-3 and C-4 proportions and provide a classification that consistently captures significant ecosystem parameters for the Great Plains.