A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum

被引：58

作者：

Freeman, J ^{[1
]}

Ward, E ^{[1
]}

Calderon, C ^{[1
]}

McCartney, A ^{[1
]}

机构：

[1] Rothamsted Res, Plant Pathogen Interact Div, Harpenden AL5 2JQ, Herts, England

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2002年 / 108卷 / 09期

基金：

英国生物技术与生命科学研究理事会;

关键词：

airborne inoculum; ascospores; Botrytis cinerea; Brassica napus; spore trapping;

D O I：

10.1023/A:1021216720024

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The development of a polymerase chain reaction (PCR) assay for the detection of inoculum of the plant pathogenic fungus Sclerotinia sclerotiorum is described. The PCR primers were designed using nuclear ribosomal DNA internal transcribed spacer sequences. Specific detection of DNA from S. sclerotiorum was possible even in the presence of a 40-fold excess of DNA from the closely related fungus Botrytis cinerea. PCR products were obtained from suspensions of untreated S. sclerotiorum ascospores alone, but DNA purification was required for detection in the presence of large numbers of B. cinerea conidiospores. Specific detection of inoculum of S. sclerotiorum was possible in field-based air-samples, using a Burkard spore trap, and from inoculated oilseed rape petals. The assay has potential for incorporation into a risk management system for S. sclerotiorum in oilseed rape crops.

引用

页码：877 / 886

页数：10

共 41 条

[1] USE OF SOLID-PHASE PCR FOR ENHANCED DETECTION OF AIRBORNE MICROORGANISMS [J].

ALVAREZ, AJ ;

BUTTNER, MP ;

TORANZOS, GA ;

DVORSKY, EA ;

TORO, A ;

HEIKES, TB ;

MERTIKASPIFER, LE ;

STETZENBACH, LD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (01) :374-376

[2] PCR FOR BIOAEROSOL MONITORING - SENSITIVITY AND ENVIRONMENTAL INTERFERENCE [J].

ALVAREZ, AJ ;

BUTTNER, MP ;

STETZENBACH, LD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (10) :3639-3644

[3] USE OF A SELECTIVE MEDIUM TO STUDY THE DISPERSAL OF ASCOSPORES OF SCLEROTINIA-SCLEROTIORUM [J].

BENYEPHET, Y ;

BITTON, S .

PHYTOPARASITICA, 1985, 13 (01) :33-40

[4] Evaluation of polyclonal-antibody-based immunoassays for detection of Sclerotinia sclerotiorum on canola petals, and prediction of stem rot [J].

Bom, M ;

Boland, GJ .

CANADIAN JOURNAL OF MICROBIOLOGY, 2000, 46 (08) :723-729

[5]

British Aerobiology Federation, 1995, Airborne Pollen and Spores: A Guide to Trapping and Counting

[6] Detection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assays [J].

Calderon, C ;

Ward, E ;

Freeman, J ;

McCartney, A .

JOURNAL OF AEROSOL SCIENCE, 2002, 33 (02) :283-296

[7]

DAVIES JML, 1986, OILSEED RAPE, P83

[8]

FITT B, 1992, AGRONOMIST, V1, P12

[9] Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp oleifera) [J].

Foster, SJ ;

Singh, G ;

Fitt, BDL ;

Ashby, AM .

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1999, 55 (02) :111-119

[10]

*GEN COMP GROUP, 1994, PROGR MAN WISC PACK

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