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Extracellular calcium modulates in vitro bone marrow-derived Flk-1+ CD34+ progenitor cell chemotaxis and differentiation through a calcium-sensing receptor

被引：95
作者：
Aguirre, A. [1 ,2 ,3 ]
Gonzalez, A. [1 ]
Planell, J. A. [1 ,2 ,3 ]
Engel, E. [1 ,2 ,3 ]
机构：
[1] Inst Bioengn Catalonia, Barcelona 08028, Spain
[2] Tech Univ Catalonia UPC, Dept Met Engn & Mat Sci, ETSEIB, Barcelona 08028, Spain
[3] CIBER BBN, Zaragoza 50118, Spain
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 393卷 / 01期
关键词：
Endothelial progenitor cell; Calcium-sensing receptor; Angiogenesis; Chemotaxis; Calcium; Bone marrow; ENDOTHELIAL-CELLS; CA2+-SENSING RECEPTOR; ANGIOGENESIS; NEOVASCULARIZATION; POPULATION; ACTIVATION; EXPRESSION; APOPTOSIS; VIVO;
D O I：
10.1016/j.bbrc.2010.01.109
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Angiogenesis is a complex process regulated by many cell types and a large variety of biochemical signals such as growth factors, transcription factors, oxygen and nutrient diffusion among others. In the present study, we found out that Flk-1(+) CD34(+) progenitor cells (bone marrow resident cells with an important role in angiogenesis) were responsive to changes in extracellular calcium concentration through a membrane bound, G-protein-coupled receptor sensitive to calcium ions related to the calcium-sensing receptor (CaSR). Calcium was able to induce progenitor cell migration in Boyden chamber experiments and tubulogenesis in Matrigel assays. Addition of anti-CaSR antibodies completely blocked the effect, while CaSR agonist Mg2+ produced a similar response to that of calcium. Real time RT-PCR for a wide array of angiogenesis-related genes showed increased expression of endothelial markers and signaling pathways involved in angiogenesis. These results suggest calcium could be a physiological modulator of the bone marrow progenitor cell-mediated angiogenic response. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：156 / 161
页数：6
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