Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)

被引：25

作者：

Denovan-Wright, EM

Pierce, M

Wright, JM ^{[1
]}

机构：

[1] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4J1, Canada

[2] Dalhousie Univ, Dept Pharmacol, Halifax, NS B3H 4H7, Canada

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1492卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

brain fatty acid binding protein; zebrafish; cDNA cloning; in situ hybridization; Northern analysis;

D O I：

10.1016/S0167-4781(00)00075-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the nucleotide sequence for two cDNA clones coding for a fatty acid binding protein (FABP) from zebrafish (Danio rerio). Comparison of the sequence with GenBank entries revealed extensive amino acid identity between this zebrafish FABP and brain FABPs (B-FABP) from other species. The zebrafish B-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases BglII or EcoRI suggesting that a single copy of the B-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish B-FABP mRNA is approximately 850 nucleotides in length. In situ hybridization revealed that the B-FABP mRNA was expressed in the periventricular gray zone of the optic tectum of the adult zebrafish brain. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：221 / 226

页数：6

共 36 条

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