Amplification of DNA sequences in polar bodies from human oocytes for diagnosis of mitochondrial disease

被引：15

作者：

Briggs, DA

Power, NJ

Lamb, V

Rutherford, AJ

Gosden, RG

机构：

[1] Univ Leeds, Div Obstet & Gynaecol, Leeds, W Yorkshire, England

[2] Leeds Gen Infirm, Leeds, W Yorkshire, England

来源：

LANCET | 2000年 / 355卷 / 9214期

关键词：

D O I：

10.1016/S0140-6736(00)02171-1

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

When a preovulatory oocyte reinitiates meiosis, it sheds a cytoplasmic fragment containing mitochondria and redundant set of chromosomes. We have detected DNA sequences from both the mitochondrial and nuclear genomes in polar bodies from unfertilised human oocytes, demonstrating the feasibility of diagnosing mitochondrial diseases before conception.

引用

页码：1520 / 1521

页数：2

共 4 条

[1] Skewed segregation of the mtDNA nt 8993 (T->G) mutation in human oocytes [J].